摘要
为筛选与目的基因连锁更加准确的分子标记以及明确分子标记鉴定技术的准确率,同时为后期进行基因精细定位研究提供技术手段,本试验采用F_(2∶3)家系进行F_2单株的TuMV抗性鉴定。结果表明,122个大白菜抗TuMV材料8407和感病材料冠291的F_(2∶3)家系中,抗病的有98个,感病的有24个,经卡方测验,符合3∶1的分离比例,与以往对该群体F_2代TuMV抗性分离比例的研究一致,表明这种鉴定方法可行。本结果可为今后分离群体单株TuMV抗性的准确鉴定提供参考。
In order to screen out molecular markers with more accurate linkage distance and make clear of the identification accuracy of molecular markers,as well as for fine gene mapping in the later period,the F 2∶3 families were used to identify the TuMV resistance of F 2 single plant.The results showed that in 122 Chinese cabbage F 2∶3 families with their resistant parent‘8407’and susceptible parent‘Guan 291’,98 were resistant families and 24 were susceptible families,which was in accordance with the 3∶1 separation ratio by Chi-square test.This was consistent with previous studies in segregation ratio of TuMV resistance in F 2 generation,which indicated that this method was feasible.This study provided a methodological guide for accurate identification of individual isolate on TuMV resistance in the future.
作者
满卫萍
张志刚
李春民
赵智中
王荣花
刘栓桃
李巧云
王立华
Man Weiping;Zhang Zhigang;Li Chunmin;Zhao Zhizhong;Wang Ronghua;Liu Shuantao;Li Qiaoyun;Wang Lihua(College of Life Sciences,Shandong Normal University,Jinan 250014,China;Vegetables and Flowers Institute,Shandong Academy of Agricultural Sciences/Shandong Branch of National Centerfor Vegetables Improvement/Shandong Key Laboratory for Biology of Greenhouse Vegetables,Jinan 250100,China;Penglai Daliuhang Comprehensive Agricultural Service Station,Penglai 265615,China)
出处
《山东农业科学》
2019年第1期115-118,共4页
Shandong Agricultural Sciences
基金
山东省重点研发计划项目"大白菜TuMV抗性基因retrcs03的定位研究"(2017GSF21103)
国家重点研发计划子课题"大白菜杂种优势群划分与强优势新品种选育"(2016YFD0101701)
山东省农业重大应用技术创新项目"大白菜轻简化制种与分子鉴定技术研究与应用"
山东省农业科学院农业科技创新工程项目"蔬菜杂种优势利用育种体系与关键技术"(CXGC2018B03)
