摘要
以传统东北酸菜为研究对象,从中分离筛选高产酸乳酸菌,通过探究乳酸菌的低温生长性能、耐盐特性及抑菌能力,从中获得优良发酵性能的乳酸菌,其经分子生物学鉴定后应用于发酵酸菜。结果表明,从传统东北酸菜分离筛选获得192株乳酸菌,其中5株为高产酸乳酸菌,均被鉴定为植物乳杆菌(Lactobacillus plantarum);5株高产酸乳酸菌中植物乳杆菌DBC3和DBC33兼有多种优良性能,且在15℃条件下,接种量为3%的植物乳杆菌DBC33发酵得到的酸菜感官评分最高,显著缩短了发酵周期(P<0.05),为东北酸菜的标准化生产和酸菜发酵剂的制备提供了参考依据。
Using traditional pickle in the northeast of China as research object,the high-yield acid lactic acid bacteria were isolated and screened.The lactic acid bacteria with superior fermentation performance were obtained by exploring the low temperature growth performance,salt tolerance and antibacterial ability,identified by molecular biology and applied in the fermented pickle.The results showed that 192 lactic acid bacteria were obtained from traditional pickle in the northeast of China,among them,5 strains were high-yield acid lactic acid bacteria,which were identified as Lactobacillus plantarum,and the L.plantarum DBC3 and DBC33 had a variety of superior fermentation properties.The sensory evaluation of the fermented pickle by L.plantarum DBC33 with inoculum 3%at 15℃was the highest,and the strain DBC33 significantly shortened the fermentation period of pickle(P<0.05).This study provided a reference basis for the standardized production of pickle in the northeast of China and preparation of pickle starter.
作者
赵山山
杜秋玲
杨晓艳
张莎莎
王磊
邸一桓
郝光飞
赵国忠
周玉岩
ZHAO Shanshan;DU Qiuling;YANG Xiaoyan;ZHANG Shasha;WANG Lei;DI Yihuan;HAO Guangfei;ZHAO Guozhong;ZHOU Yuyan(College of Life Science and Food Engineering,Hebei University of Engineering,Handan 056000,China;Key Laboratory of Food Nutrition and Safety,Ministry of Education,College of Food Engineering and Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China;Hebei Provincial Institute for Drug Control,Shijiazhuang 050011,China)
出处
《中国酿造》
CAS
北大核心
2019年第1期42-48,共7页
China Brewing
基金
河北省自然科学基金资助项目(C2016402014)
河北省高等学校科学技术研究项目(QN2017033)
邯郸市科学技术研究与发展计划项目(1622201049)
关键词
东北酸菜
高产酸乳酸菌
发酵性能
筛选
应用
pickle in the northeast of China
high-yield acid lactic acid bacteria
fermentation property
screening
application
