摘要
该研究采用海藻酸钙凝胶法、琼脂凝胶法、明胶-戊二醛交联法和聚乙烯醇(PVA)包埋法对酿酒酵母(Saccharomyces cerevisiae)1308进行固定,通过4种固定方法的比较,选择低成本、性能优越的固定化方法。在此基础上,进一步优化PVA固定化条件,并将PVA固定化酵母用于分批发酵和连续发酵。结果表明,PVA包埋法为最佳固定化方法,PVA最优添加量为8%;8%PVA固定化酵母生物具有较好的稳定性,分批发酵其稳定使用期>164 d,可连续重复使用82批次以上;连续发酵的稳定性>4个月;其在分批发酵和连续发酵应用方面,乙醇生产能力和稳定性均优于游离酵母细胞。
Saccharomyces cerevisiae 1308 was immobilized by calcium alginate gel,agar gel,gelatin-glutaraldehyde cross-linking and polyvinyl alcohol(PVA)methods.The immobilization method with low-cost,superior performance was chosen through the comparison of four kinds of immobilization methods.On the basis,the immobilization condition of PVA method was further optimized,and the immobilized yeast was used for batch and continuous fermentation.The results showed that the PVA method was the optimal immobilization method,and the optimal PVA addition was 8%.The 8%PVA-immobilized yeast had good stability,and the stable usage period of 8%PVA-immobilized yeast for batch fermentation was more than 164 d,which could be continuously reuse for more than 82 batches;the stability of continuous fermentation was more than 4 months.The ethanol production capacity and stability of the 8%PVA-immobilized yeast were superior to free yeast cells in batch fermentation and continuous fermentation applications.
作者
祝英
庄鹤桐
王治业
周剑平
ZHU Ying;ZHUANG Hetong;WANG Zhiye;ZHOU Jianping(Key Laboratory of Microbial Resources Exploitation and Utilization in Gansu Province,Lanzhou 730000,China;Institute of Biology,Gansu Academy of Sciences,Lanzhou 730000,China;Xiaoshan Middle School of Hangzhou City in Zhejiang Province,Hangzhou 311201,China)
出处
《中国酿造》
CAS
北大核心
2019年第1期113-117,共5页
China Brewing
基金
甘肃省基础创新团队项目(1606RJIA325)
甘肃省重点基金项目(18JR3RA254)
甘肃省科学院创新团队项目(CX201601)
甘肃省科学院优秀青年基金项目(2016YQ-02)
甘肃省科学院应用开发项目(2018JK-04&2018JK-15)
中国科学院西部之光项目
关键词
固定化方法
酿酒酵母
聚乙烯醇
乙醇生产能力
发酵
immobilization method
Saccharomyces cerevisiae
polyvinyl alcohol
ethanol production capacity
fermentation
