摘要
目的探讨B7-H3和癌胚抗原(CEA)在恶性胸腔积液(MPE)诊断中的价值。方法收集40例MPE和22例良性胸腔积液(BPE)标本,应用酶联免疫吸附试验(ELISA)法测定B7-H3和CEA在MPE和BPE中的表达,绘制受试者工作曲线(ROC曲线),计算相应曲线下面积(AUC)、确定诊断MPE灵敏度、特异度及诊断界值。结果 B7-H3诊断MPE的相应灵敏度、特异度为62.5%、81.0%,AUC为0.777;CEA诊断MPE的相应灵敏度、特异度为72.5%、81.0%,AUC为0.850;二者联合诊断的灵敏度、特异度为70.0%、98.2%,AUC为0.910。结论 B7-H3和CEA可作为MPE的诊断指标,二者联合应用诊断价值更高,有望在未来临床中诊断中应用。
Objective To investigate the diagnostic value of B7-H3 and carcinoembryonic antigen(CEA)on diagnosis of malignant pleural effusion(MPE).Methods We collected and analysed the expression of B7-H3 and CEA in 40 MPE cases and 22 cases of benign pleural effusion(BPE)using enzyme-linked immunosorbent assay(ELISA).Receiver operator characteristic curves(ROC)were drawn according to the expression of B7-H3 and CEA,calculated diagnosis sensitivity,specificity and the area under curve(AUC).Results The diagnosis sensitivity of B7-H3 was 62.5%,specificity 81.0%,with the AUC of 0.777;similarly,The diagnosis sensitivity of CEA was 72.5%,specificity 81.0%,with the AUC of 0.850.Higher AUC of 0.910 was gained in combination ROC,with sensitivity and specificity of 72.5%,81.0%,respectively.Conclusion B7-H3 and CEA could be available diagnosing markers for MPE.Combined applications of B7-H3 and CEA have higher AUC.They may be widely applied in future clinical practice.
作者
胡琼英
罗建蓉
艾承锦
陈高莉
张朝明
熊大迁
HU Qiongying;LUO Jianrong;AI Chengjin;CHEN Gaoli;ZHANG Chaoming;XIONG Daqian(Department of Laboratory Medicine,Teaching Hospital of Chengdu University of T.C.M,Chengdu 610072,China;Department of Laboratory Medicine,Qionglai Medical Center Hospital,Qionglai 611530,China)
出处
《国际检验医学杂志》
CAS
2019年第3期266-268,共3页
International Journal of Laboratory Medicine
基金
国家自然科学基金(81601835)
四川省科技厅面上项目(18YYJC0689)
中华医学会教育项目(2016B-FF095)
四川省医学会科研项目(S15023)
成都中医药大学青年基金(ZRQN1730
030038065)
关键词
恶性胸腔积液
良性胸腔积液
B7-H3
癌胚抗原
Malignant pleural effusion
Benign pleural effusion
B7-H3
Carcinoembryonic antigen
