摘要
目的了解重庆地区金黄色葡萄球菌临床菌株的分子型别及其对常用抗菌药物的耐药情况,为防控该地区金葡菌感染提供理论依据。方法收集2013年6月至2014年12月期间重庆地区西南医院临床分离的110株金黄色葡萄球菌,利用特征性基因扩增区分甲氧西林敏感金黄色葡萄球菌(MSSA)和耐甲氧西林金黄色葡萄球菌(MRSA),再采用多位点序列分型(MLST)、spa分型、SCCmec分型、agr分型等方法检测金葡菌的型别,采用PCR检测pvl毒力基因,药敏试验分析菌株对苯唑西林、万古霉素、四环素等13种临床常用抗菌药物的耐药性,分析菌株的耐药谱。结果 110株金葡菌中有59株为MRSA,51株为MSSA,MRSA阳性率为53.6%。59株MRSA分为11种spa型和8种ST型,优势流行克隆为ST239-MRSA-Ⅲ(57.6%,34/59),pvl毒力基因检出率为23.7%(14/59)。51株MSSA分为27种spa型和18种ST型,agrI型占68.6%(35/51),pvl检出率为7.8%(4/51)。药敏结果显示59株MRSA均为多重耐药(MDR)菌株,51株MSSA中有24株为MDR菌株。结论重庆地区流行的MRSA以ST239-MRSA-Ⅲ为主,而MSSA表现出较高遗传多样性,发现ST121等高毒力、高耐药性菌株,值得高度重视。
Objective To detect the molecular types and drug susceptibilities of Staphylococcus aureus strains isolated in Chongqing.Methods A total of 110 S.aureus isolates were collected between June 2013 and December 2014 from Southwest hospital in Chongqing.The methicillin-susceptible S.aureus(MSSA)and methicillin-resistant S.aureus(MRSA)were determined.The molecular typing methods such as multilocus sequence typing(MLST),staphylococcal protein A(spa)gene typing,staphylococcal cassette chromosome mec(SCCmec)typing,and accessory gene regulator(agr)typing were applied for the strains.The carriage of Panton-Valentine Leukocidin gene(pvl)and drug susceptibilities of S.aureusstrains against 13 common used antibiotics were determined.Results In the 110 S.aureus isolates,59 were MRSA(53.6%)and 51 were MSSA(46.4%).The MRSA strains were divided into 11 spa types and 8 ST types,ST239-MRSA-Ⅲwas the most prevalent clone(57.6%,34/59).The frequency of pvl carriage was 23.7%(14/59)in MRSA strains.Among 51 MSSA strains,27 spa types and 18 ST types were found.Most MSSA strains were agr I types(68.6%,35/51).The pvl carriage was 7.8%(4/51).All MRSA strains were multidrug resistant(MDR),whereas 24 out of the 51 MSSA strains(47.1%)belonged to MDR.Conclusion ST239-MRSA-Ⅲis the most predominant MRSA in Chongqing.MSSA isolates show wide genetic diversity.The MSSA isolates show high resistance to multiple antibiotic drugs,which indicate that efforts to fight infections caused by MSSA in certain region,such as Chongqing need to be intensified.
作者
林君
曹利
LIN Jun;CAO Li(Department of Clinical Laboratory,the Seventh People′s Hospital,Chongqing 400054,China;Department of Clinical Laboratory,Southwest Hospital of Army Military Medical University,Chongqing 400038,China)
出处
《国际检验医学杂志》
CAS
2019年第3期342-347,共6页
International Journal of Laboratory Medicine
关键词
金黄色葡萄球菌
分子分型
耐药谱
多重耐药
Staphylococcus aureus
molecular typing
drug susceptibility testing
multidrug-resistance
