摘要
采用不同培养基从分离自自然发酵豆酱样品中的285株乳酸菌种中分离筛选出3株能够耐受人工胃肠环境,具有降胆固醇作用的乳酸菌,编号分别为DQ17-2、DD1-1、KY1-1,对胆固醇的去除率分别为34. 56%、27. 12%和29. 20%,最后通过形态学观察、生理生化试验、16S r DNA序列鉴定法确定3株乳酸菌均为植物乳杆菌。将菌株DQ17-2重新命名为植物乳杆菌WW(L. plantarum WW),为了进一步验证其在体内的降胆固醇作用,将32只5周龄雄性SD大鼠随机分为4组:普通对照组(A)、高脂模型组(B)、脱脂乳对照组(C)、菌液干预组(D)。饲喂12周后,采血,测定血清总胆固醇(totalcholesterol,TC)、高密度脂蛋白胆固醇(highdensity lipoprotein-cholesterol,HDL-C)、低密度脂蛋白胆固醇(lowdensity lipoprotein-cholesterol,LDL-C)、甘油三酯(triglyceride,TG)含量。结果表明,饲喂12周后成功构建高脂模型,与B组和C组相比,D组血清TC、TG、LDL-C含量明显降低(P <0. 05),HDL-C含量明显升高(P <0. 05)。该实验筛选出一株在体内外均具有降胆固醇作用的L. plantarum WW,为今后降胆固醇产品的研发提供原料与依据。
Three lactic acid bacteria strains,DQ17-2,DD1-1 and KY1-1,being resistant to the artificial gastrointestinal environment and cholesterol-lowering effect(the cholesterol removal rates were 34.56%,27.12%and 29.20%,respectively),were selected from 285 isolates of lactic acid bacteria,which were isolated from the naturally fermented soybean sauces using three different media.They were identified to be Lactobacillus plantarum by morphology,physiological and biochemical tests and 16S rDNA sequencing.The strain DQ17-2 was renamed as In order to further verify its in vivo cholesterol-lowering effect,L.plantarum WW cell suspends were fed 32 5-week-old male SD rats within randomly 4 groups[Control group(A),High fat group(B),Skim milk control group(C)and L.plantarum WW fed group(D)].After feeding for 12 weeks,the levels of total cholesterol(TC),high density lipoprotein-cholesterol(HDL-C),low density lipoprotein-cholesterol(LDL-C)and triglyceride(TG)in serum were examined.The results showed that the model of hyperlipidemia was successfully established and the levels of TC,TG,and LDL-C in group D were significantly lower than those of group B and C(P<0.05)and the level of HDL-C was significantly increased(P<0.05).In conclusion,L.plantarum WW had both in vivo and in vitro cholesterol-lowering effect,which provides a new strategy for development of cholesterol-lowering products.
作者
郭晶晶
张鹏飞
曹承旭
邹婷婷
乌日娜
GUO Jingjing;ZHANG Pengfei;CAO Chengxu;ZOU Tingting;WU Rina(College of Food Shenyang Agricaltural University,Shenyang 110866,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2019年第2期15-24,共10页
Food and Fermentation Industries
基金
国家自然科学基金面上项目(31471713)
辽宁省高等学校优秀人才支持计划项目(LR2015059)
辽宁省"百千万人才工程"人选科技活动支持项目
沈阳市高层次科技创新人才(RC70212)
关键词
植物乳杆菌
降胆固醇
筛选
鉴定
大鼠
Lactobacillus plantarum
cholesterol lowering
screening
identification
rats
