摘要
以南极磷虾为研究对象,采用丙酮提取南极磷虾中的虾青素。优化硅胶柱层析技术分离虾青素双酯、单酯及游离虾青素;筛选合适的展开剂,利用薄层层析法对得到的样品进行鉴定;通过Chiralpak IC柱鉴别其立体结构;运用C_(30)-HPLC法测定不同形态虾青素的含量。结果表明,硅胶柱层析以不同比例的丙酮-石油醚溶液梯度淋洗,可以分离制备虾青素双酯、单酯及游离虾青素; V(正己烷)∶V(丙酮)∶V(乙酸)=8∶2∶0. 2为薄层层析最佳展开剂。南极磷虾中不同形态虾青素均由3S,3'S、3S,3'R、3R,3'R 3种光学异构体组成,虾青素双酯和单酯中3R,3'R所占比例> 70%,3S,3'S的比例最低。南极磷虾中总虾青素含量为18. 9 mg/kg,其中虾青素双酯中虾青素含量为12. 0 mg/kg,虾青素单酯中虾青素含量为6. 1 mg/kg,游离虾青素含量为0. 8 mg/kg。
In this study,acetone was used to extract the astaxanthin from Antarctic krill.The silica gel column chromatography was optimised for separating astaxanthin diester,astaxanthin monoester,and free astaxanthin.A suitable developing solvent was selected.Samples were identified by a thin layer chromatography and the three-dimensional structures were confirmed by Chiralpak IC column.The contents of astaxanthin in different forms were quantified using C30-HPLC.The results showed that a gradient elution through silica gel column chromatography with different ratios of acetone-petroleum ether could completely separate free astaxanthin,astaxanthin monoester and diester.V(n-hexane)∶V(acetone)∶V(acetic acid)=8∶2∶0.2 was the optimum developing solvent for thin layer chromatography.Astaxanthin from Antarctic krill included three optical isomers:3S,3′S,3S,3′R,and 3R,3′R.The proportions of 3R,3′R in astaxanthin diesters and monoesters were more than 70%,and the proportion of 3S,3′S was the lowest.The total astaxanthin content in Antarctic krill was 18.9 mg/kg,of which the astaxanthin contents of astaxanthin diester,astaxanthin monoester,and free astaxanthin were 12.0,6.1 and 0.8 mg/kg,respectively.
作者
丛心缘
孙伟红
张辉珍
冷凯良
邢丽红
苗钧魁
刘小芳
CONG Xinyuan;SUN Weihong;ZHANG Huizhen;LENG Kailiang;XING Lihong;MIAO Junkui;LIU Xiaofang(Qingdao University,School of Public Health,Qingdao 266071,China;Yellow Sea Fisheries Research Institute,ChineseAcademy of Fishery Sciences,Key Laboratory of Sustainable Development of Polar Fishery,Ministry of Agriculture,Qingdao266071,China;Qingdao Food and Drug Administration,Qingdao 266071,China;Laboratory for Marine Drugs andBioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266071,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2019年第2期174-178,共5页
Food and Fermentation Industries
基金
中国水产科学研究院基本科研业务费专项(2016HY-ZD0902)资助
关键词
南极磷虾
虾青素酯
硅胶柱层析
光学异构体
Antarctic krill
astaxanthin ester
silica gel column chromatography
optical isomers
