摘要
目的:优化广玉兰果实的有效成分双和厚朴酚A的提取工艺。方法:采用反相高效液相色谱法测定不同提取溶媒中双和厚朴酚A的含量。以双和厚朴酚A得率为指标,采用L9(34)正交试验考察料液比、浸泡时间及提取次数3个因素对提取工艺的影响。结果:双和厚朴酚A的最佳提取工艺条件是正己烷为提取溶媒,料液比为1∶30、浸泡时间为48 h、提取次数为3次。结论:通过正交设计法优选的双和厚朴酚A提取工艺简单、稳定可靠、重复性好。
Objective:To optimize the extraction technology of bishonokiol A from the Magnolia grandiflora seeds.Methods:The concentration of bishonokiol A in different extracting solvents was determined using RP-HPLC method.The yield rate of bishonokiol A was set as the indictor,the effects of the ratio of material-to-liquid,soaking duration,and extraction times on the extraction technology were observed using L9(34)orthogonal test.Results:The orthogonal test showed that the ratio of material-to-liquid for 1∶30,soaking duration for 48 h,and extraction times for three times were the best technological condition of bishonokiol A extraction.Conclusions:The optimized extraction process designed by orthogonal test is simple,stable and reliable.
作者
张松松
靳伟
李红梅
吴成柱
ZHANG Song-song;JIN Wei;LI Hong-mei;WU Cheng-zhu(School of Pharmacy,Bengbu Medical College,Bengbu Anhui 233030,China)
出处
《蚌埠医学院学报》
CAS
2019年第1期89-92,共4页
Journal of Bengbu Medical College
基金
国家自然科学基金项目(81302671)
安徽省大学生创新创业训练项目(AH201410367051)
关键词
双和厚朴酚A
广玉兰
正交设计
bishonokiol A
Magnolia grandiflora
orthogonal design
