摘要
选择20只舍饲肢体病辽宁绒山羊母羊,随机分成5组,其中1组为对照组,2～5组为试验组,各组每只羊依次补饲蛋氨酸锌0、342、515、686、857 mg/d(折算为锌分别是0、60、80、120、150 mg/d)。饲养60 d后,采集静脉血样测定营养代谢指标、抗氧化指标和骨关节损伤生物学标志物,试验结束时连续3 d观察试验羊肢蹄状况。结果表明:随着蛋氨酸锌补饲量的增加,血清INS和PG含量均呈现降低趋势,其中4、5组的INS和PG显著(P<0.05)低于对照组,血糖呈增加趋势,其中4、5组显著(P<0.05)高于1组。血清UA含量呈降总体低趋势;血清UN水平呈总体增加趋势,试验5组显著(P<0.05)高于对照组。血清GSH-PX、SOD、CAT、GSSG活性呈增加趋势,其中4、5组GSH-PX、CAT、GSSG显著(P<0.05)高于对照组,4、5组的SOD和CAT活性极显著(P<0.01)高于对照组;血清MDA呈降低趋势。血清COMP、CTX-Ⅱ和PⅡANP含量都呈降低趋势,其中5组COMP、CTX-Ⅱ的含量显著(P<0.05)低于对照组。经观察发现补饲蛋氨酸锌后肢蹄病羊的关节肿大、关节僵直、跛行、跪地膝行等症状得到明显改善。结论:在舍饲肢蹄病羊日粮中添加120～150 mg/d蛋氨酸锌显著提高了机体抗氧化能力,保护了关节软骨免受损害,对促进肢蹄病羊恢复具有明显作用。
Twenty Liaoning Cashmere female goats with limp hoof disease were randomly divided into five groups, and the first group was the control, the 2nd to 5th were fed with Zn-Met 0 mg/d, 342.9 mg/d, 514.3 mg/d, 685.7 mg/d and 857.1 mg/d(Converted into zinc means 0 mg/d,60 mg/d, 80mg/d, 120 mg/d, 150 mg/d). All female goats were feed 60 days, and serum indicators about glucose and nitrogen metabolic, antioxidant stressenzymes, biological markers of bone and joint injuries were determinated, the conditions of Limb and foot before and after test were observed and re‐corded. The results showed that with increasing of Zn-Met, Serum INS and PG were decreasing, and they were significantly(P<0.05) lower in group 4 and 5(120 mg/d^150 mg/d) than the control;se‐rum Glucose was increasing, and it was significantly(P<0.05) higher in group 4 and 5 than the control. Serum UA was decreasing and UN was incresing. Activity of GSH-PX, SOD, CAT, GSSG were all increasing, and GSH-PX, CAT, GSS in group 4 and 5 were all higher than the control;while MDA was decreasing. Serum COMP, CTX-Ⅱ and PⅡANP were all decreasing, and COMP, CTX-Ⅱ in in group 5 were lower than the control. It was observed that Zn-Met could improved knee joints health such as swelling, stiff and limp or fall on knees with pain. So it was concluded that feeding the Cashmere goats with Zn-Met could improve activity of antioxidant stress, protect bone and joint and be benefit to recover fromp limp hoof disease when Liaoning Cashmere Goat fed in house, and the best level of Zn-Met was 120 mg/d^150 mg/d.
作者
孙亚波
Sun Yabo(The Institute of Animal Husbandry of Liaoning Province,Liaoning Liaoyang 111000)
出处
《现代畜牧兽医》
2019年第1期17-22,共6页
Modern Journal of Animal Husbandry and Veterinary Medicine
基金
辽宁省农业攻关及产业化"舍饲绒山羊营养代谢病及副结核病防控技术"(2015103036)
"辽宁省羊产业科技创新团队"
国家绒毛用羊产业技术体系"饲料资源开发岗位"项目(CARS-CARS-39-10)支持
关键词
蛋氨酸锌
抗氧化
辽宁绒山羊
肢蹄病
Zn-Met
Antioxidant stress
Liaoning Cashmere Goat
Limp Hoof disease
