摘要
为了解鸡群中I群禽腺病毒(FAd V-I)的流行现状,从山东、黑龙江、吉林等地部分大规模养鸡场采集病料,进行病毒分离、PCR鉴定及同源性分析。结果显示:从76份病料中分离到10株FAd V-I,分离率为13%;10株FAd V-I中,1株为血清4型,6株为血清8b型,1株为血清9型,2株为血清11型。结果表明,山东、黑龙江、吉林等地均存在一定的FAd V-I流行,主要流行血清型已由4型转变为8b型,因此需要评估当前的4型灭活苗能否有效防控这些地区的FAd V-I流行。
In order to recognize the prevalence of group I fowl adenovirus(FAdV-I)in poultry,samples were collected from several large-scale chicken farms in Shandong,Heilongjiang and Jilin provinces for FAdV isolation,PCR assay and homology analysis.Results showed that 10 FAdV-I strains were isolated from 76 samples with the positive rate reaching 13%.Among the 10 FAdV-I strains,1 of them belonged to serotype 4, 6 of them belonged to serotype 8b,1 of them belonged to serotype 9,and 2 of them belonged to serotype 11.The results indicated that certain FAdV-I infection existed in Shandong,Heilongjiang and Jilin provinces, and the main serotypes had been transferred from serotype 4 to serotype 8b,thus the current serotype 4 inactivated vaccines need to be evaluated to determine if they could control the spread of FAdV-I effectively.
作者
矫海婷
张灿
刘德萍
宋翠萍
李东阳
任慧英
吴延功
Jiao Haiting;Zhang Can;Liu Deping;Song Cuiping;Li Dongyang;Ren Huiying;Wu Yangong(Qingdao Agricultural University,Qingdao,Shandong 266109,China;China Animal Health and Epidemiology Center,Qingdao,Shandong 266032;Chengxi Animal Husbandry and Veterinary Station,Rongcheng,Shandong 264300,China)
出处
《中国动物检疫》
CAS
2019年第2期58-62,共5页
China Animal Health Inspection
基金
国家重点研发计划项目(2018YFD0501400)
关键词
I群禽腺病毒
分离鉴定
同源性
血清型
group I fowl adenovirus
isolation and identification
homology
serotype
