摘要
目的探讨甘草黄酮对高糖刺激下人视网膜色素上皮细胞(RPE)氧化应激的影响。方法采用40 mmol·L^(-1)的葡萄糖培养RPE细胞建立细胞氧化损伤模型,用10^(-9)mol/L、10^(-8)mol/L浓度的甘草黄酮干预,观察细胞活性、细胞内活性氧(ROS)水平、凋亡细胞比例及凋亡相关蛋白表达的变化。结果 MTT试验结果显示,用10-9mol/L、10-8mol/L浓度的甘草黄酮处理后,RPE细胞存活率分别提高到67.86%±3.23%和78.67%±2.57%,与高糖组(40.45%±3.27%)比较,差异具有统计学意义(P<0.01);2',7'-二氯二氢荧光素二乙酸酯(H2DCFDA)荧光探针染色结果显示,甘草黄酮可不同程度抑制40 mmol/L葡萄糖处理后RPE细胞内ROS水平;此外,甘草黄酮可以抑制高糖诱导的RPE细胞凋亡及细胞内Caspase-3及Caspase-9的表达,其抑制效应与浓度呈正相关。结论甘草黄酮可以抑制高糖诱导的人眼RPE细胞氧化损伤及凋亡的发生,对糖尿病性视网膜病变具有预防作用。
OBJECTIVE To investigate the effect of licorice flavonoids on high glucose-induced human retina pigment epithelium(RPE)cells under oxidative stress.METHODS The oxidative damage model of RPE cells was induced by 40 mmol·L^-1 glucose,and intervened with different concentrations of licorice flavonoids,cell survival rate was detected by MTT colorimetry,intracellular reactive oxygen species(ROS)were detected by H2DCFDA staining,nucleus apoptosis was observed by Hoechst staining,the expression of caspase-3 and caspase-9 were tested by Western blot.RESULTS The MTT results showed that RPE cell survival rate increased to 67.86%±3.23%and 78.67%±2.57%after treated with 10^-9 mol·L^-1,10^-8 mol·L^-1 licorice flavonoids,the difference was statistically significant when compared with result(40.45%±3.27%)of high glucose group(P<0.01);The H2DCFDA fluorescent probe dying showed that licorice flavonoids reduced ROS generation in RPE cells and decreased oxidative-damaged cells;Besides,licorice flavonoids inhibited RPE cells apoptosis and the expression of high glucose-induced caspase-3 and caspase-9 expression in RPE cells,the inhibitory effect was positively correlated with the dose.CONCLUSIONS Licorice flavonoids could inhibit high glucose-induced oxidative damage and apoptosis in human RPE cells,which had preventive effect on diabetic retinopathy.
作者
王鹤
厉新新
孙晓会
吴红男
冯卓蕾
刘丽娟
WANG He;LI Xinxin(First Affiliated Hospital of Harbin Medical University,Heilongjiang 150001,China)
出处
《中国中医眼科杂志》
2019年第1期10-13,18,共5页
China Journal of Chinese Ophthalmology
基金
国家自然科学基金-青年项目(81500731)
关键词
甘草黄酮
高糖
视网膜色素上皮细胞
凋亡
licorice flavonoids
high glucose
human lens epithelium cells
apoptosis
