摘要
旨在应用基于p113基因特异性的PCR方法对绵羊肺炎支原体(Mycoplasma ovipneumoniae,Mo)安徽流行株进行分子生物学鉴定。利用已报道的Mo p113基因引物,采用PCR方法对前期分离的Mo安徽流行株AH-01、AH-02、AH-03和AH-04进行p113基因扩增,并对菌株的p113基因扩增产物进行序列测定及分析。结果表明,利用建立的PCR方法,4株Mo临床分离株均可扩增到大小约为287 bp的特异性目的片段;安徽流行株AH01、AH02、AH03和AH04的p113基因序列两两之间的相似性均在95%以上,与Mo ATCC 29419和Mo贵州流行株GZ-QX1的p113基因序列相似性在88.6%～89.3%。提示基于p113基因的PCR方法可以用于绵羊肺炎支原体的分子生物学鉴定,为开展由Mo引起的绵羊和山羊肺炎的流行病学调查和科学防控提供了新方法。
This study was designed to carry out the molecular identification of clinical isolates of Mycoplasma ovipneumoniae(Mo)prevalent in Anhui Province by using a specific PCR assay targeting at p113 gene.PCR assay was used to amplify the target gene from the four previously isolated Mo strains,AH-01,AH-02,AH-03 and AH-04,using the reported specific primers for p113 gene.Subsequently,the amplified products were sequenced and genetically analyzed.The results showed that the specific PCR products with size of 287 bp were obtained from all of the four clinical isolates;the pairwise sequence similarity of p113 gene of the four Mo isolates were all above 95%,and their sequence similarity with Mo standard strain of ATCC 29419 and Mo clinical strain of GZ-QX1 isolated from Guizou Province ranged from 88.6% to 89.3%.Our results demonstrated that the PCR assay targeting at p113 gene could be used in the molecular identification of Mo clinical isolates,which provided a methodological reference for the epidemiological investigation and scientific control for the respiratory tract infectious diseases in sheep and goat associated with Mo.
作者
侯宏艳
张丹俊
赵瑞宏
周学利
HOU Hong-yan;ZHANG Dan-jun;ZHAO Rui-hong;ZHOU Xue-li(Anhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering,Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences,Hefei 230031,China)
出处
《畜牧与饲料科学》
2019年第1期101-103,共3页
Animal Husbandry and Feed Science
基金
安徽省农业科学院学科建设项目(16A0413)
