摘要
本文以二倍体和三倍体虹鳟(Oncorhynchus mykiss)幼鱼为实验对象,探究急性高温胁迫下,不同倍性虹鳟热休克蛋白家族(hsps)基因表达水平。选取二倍体和三倍体虹鳟幼鱼,分别进行常温处理(14℃)和高温胁迫(22℃),并在高温胁迫48h后,将虹鳟置于常温(14℃)恢复48h。分别在实验开始的0,12,24,48,72和96h进行取样,测定实验虹鳟肝脏和肌肉组织中的hsp70s(hsp70a,hsp70b),hsp90s (hsp90a1a,hsp90a2a,hsp90a1b,hsp90a2a,hsp90b1,hsp90b2)mRNA水平。结果表明:hsp70s和hsp90s的各个亚型均能对热胁迫做出反应,并具有应答幅度的组织特异性和时间特异性。在胁迫6和24h后,上述基因mRNA水平达到最高,随后降低。三倍体幼鱼肝脏和肌肉hsps mRNA表达水平的峰值显著高于二倍体幼鱼。研究结果显示,三倍体虹鳟对高温环境更为敏感,需要消耗更多的能量用于适应高温环境。
In this study,we used diploid and triploid juvenile rainbow trout(Oncorhynchus mykiss)to investigate the expression patterns of heat shock protein family(hsps)related genes under acute hyperthermia.The diploid and triploid rainbow trout juveniles were divided into normal(14 ℃)and high temperature(22 ℃)groups for heat stressing.The high water temperature stressing lasted for 48 hours and then the temperature was decreased to the normal.Expression levels of hsp70s(hsp70a,hsp70b)and hsp90s(hsp90a1a,hsp90a2a,hsp90a1b,hsp90a2a,hsp90b1,hsp90b2)in liver and muscle were detected through qRT-PCR.After heat stressing,muscle and hepatic hsp70s and hsp90 expression levels peaked at 6 hours and 24 hours,respectively,and then decreased significantly.The highest hsps expression level in triploid juvenile was significantly higher than that in diploid juvenile liver and muscle.All these results indicated that triploid trout needs more energy to resist the environment stimuli.
作者
辛苑茹
温海深
李吉方
侯志帅
张美昭
车德钊
陈落落
XIN Yuan-Ru;WEN Hai-Shen;LI Ji-Fang;HOU Zhi-Shuai;ZHANG Mei-Zhao;CHE De-Zhao;CHEN Luo-Luo(The Key Laboratory of Mariculture(Ocean University of China),Ministry of Education,Qingdao 266003,China)
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2019年第3期129-137,共9页
Periodical of Ocean University of China
基金
山东省重点研发计划(产业关键技术)项目(2016CYJS04A01)
山东省农业良种工程项目(2016LZGC003)资助~~
关键词
虹鳟
二倍体
三倍体
热休克蛋白
基因表达
高温胁迫.
rainbow trout
diploid
triploid
heat shock protein
gene expression
high temperature stress
