摘要
目的:研究罗勒30%、50%、80%醇沉多糖对体外抗菌及免疫活性的影响,确定罗勒多糖的有效部位。方法:水提醇沉制备罗勒粗多糖,将罗勒粗多糖3次醇沉后去蛋白得精多糖,再分级醇沉得到30%、50%、80%醇沉多糖,最终测定其体外抗菌及免疫活性。结果:30%醇沉多糖具有最佳的抑菌效果和最高的刺激巨噬细胞增殖能力; 50%醇沉多糖促进脾脏细胞增殖作用最为显著。结论:不同浓度的醇沉罗勒多糖抑菌和刺激免疫细胞增殖活性有显著差异。
Objective To study the effects of 30%,50%and 80%alcohol precipitation on the antibacterial and immunological activities of Ocimum basilicum polysaccharide in vitro,and determine it s effective fraction.Method The polysaccharides of Ocimum basilicum were prepared by water extraction and alcohol precipitation.The polysaccharides were precipitated after three times alcohols of total polysaccharide,and then 30%,50%and 80%alcohol precipitated polysaccharides were obtained by fractionation and alcohol.The antibacterial and immunization activity in vitro was determined.Result 30%alcohol precipitate polysaccharide showed the best bacteriostasis effect and the highest ability to stimulate macrophage proliferation,and 50%alcohol precipitating polysaccharide promoted the proliferation of spleen cells most significantly.Conclusion Ethanol precipitated Ocimum basilicum polysaccharides with different concentrations has significant difference in bacteriostasis and immune activities.
作者
王萍
贾晓顺
朱庆均
张颖颖
WANG Ping;JIA Xiao-shun;ZHU Qing-jun;ZHANG Ying-ying(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;College of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250355,China)
出处
《中国食物与营养》
2019年第1期63-66,共4页
Food and Nutrition in China
基金
国家重大新药创制子课题(项目编号:2017ZX09301030-005)
山东中医药大学研究生教改课题(项目编号:DS2017001)
关键词
罗勒多糖
分级醇沉
抑菌
免疫活性
Ocimum basilicum polysaccharide
step alcohol precipitation
bacteriostasis
immunological activity
