摘要
本试验旨在研究长途运输应激对驴血液指标的影响。试验以12头体重(140.8±5.2)kg的德州驴为研究对象,运输距离约950km,发车地气温为-20℃,目的地气温为-10℃,历时21h,其中包括10次停车观察(≤20min)。分别在运输前、运输后0h和运输后3、5、7、9、15d采集颈静脉血15mL,进行血液激素指标、血常规指标和血液生化指标检测,并分别记录运输前、运输后0h和运输后10、20、30d体重。结果显示,与运输前相比,落地后0h血清中促肾上腺皮质激素(ACTH)、热休克蛋白90(HSP90)和皮质醇(cortisol)浓度均显著升高(P<0.05),落地后3、5、7、9、15d内逐渐降低,ACTH和HSP90基本恢复至运输前水平;中性粒细胞总数(Neu)、红细胞总数(RBC)和血红蛋白浓度(HGB)均较运输前显著上升(P<0.05),白细胞总数(WBC)有所上升但与运输前差异不显著(P>0.05),淋巴细胞总数(LYM)较运输前显著下降(P<0.05);血清总蛋白(TP)、血清白蛋白(ALB)、肌酐(CREA)、乳酸脱氢酶(LDH)、运输前后均未发生显著变化(P>0.05),谷草转氨酶(AST)、肌酸激酶(CK)、尿素(UREA)均较运输前显著上升(P<0.05);体重较运输前显著下降(P<0.05),运输后10d内体重已恢复至运输前水平。上述结果表明,长途运输可诱发驴的应激反应、肝脏损伤、机体代谢水平升高等,但免疫系统未受到明显损害,在落地后15d内机体逐渐从应激反应中恢复至运输前状态。
This study was designed to investigate the effects of long-distance transport stress on blood parameters of donkey.The study was conducted with 12 heads of Dezhou donkeys with weight of(140.8±5.2)kg,the transportation distance was about 950 km,the departure temperature was-20℃,and the destination temperature was-10℃,which lasted for 21 h,including 10 parking observations(≤20 min).15 mL of jugular venous blood was collected before loading,0 h after transportation and 3,5,7,9 and 15 dafter transportation,and blood hormone indexes,blood routine indexes and blood biochemical indexes were detected,and the weights before transport,0 h after transport and 10,20 and 30 dafter transport were recorded.The results showed that theconcentrations of adrenocorticotropic hormone(ACTH),heat shock protein 90(HSP90)and cortisol in the serum at 0 hafter landing were significantly higher than those before transportation(P<0.05),and gradually decreased within 3,5,7,9 and 15 dafter transportation,and ACTH and HSP90 recovered to pre-carriage levels.The number of neutrophils(Neu),the total number of red blood cells(RBC)and the hemoglobin concentration(HGB)increased significantly after 3,5,7,9 and 15 d(P<0.05),the total number of white blood cells(WBC)increased but not significantly different from that before transportation(P >0.05),the total number of lymphocyte(LYM)was significantly lower than before transport(P<0.05).There were no significant changes in serum total protein(TP),serum albumin(ALB),creatinine(CREA)and lactate dehydrogenase(LDH)before and after transport(P >0.05).Aspartate aminotransferase(AST),creatine kinase(CK)and urea(UREA)were significantly higher than before transport(P<0.05).The body weight decreased significantly(P<0.05),and recovered to the pre-transport level within 10 dafter transportation.The above results indicated that long-distance transport induced stress response to sputum,liver damage and elevated levels of metabolism,but the immune system was not significantly damaged.Within 15 dafter landing,the body gradually recovered from the stress response to the pre-shipment state.
作者
高维平
姜桂苗
梁伟超
李俊桥
嵇传良
赵付伟
邓立新
GAO Weiping;JIANG Guimiao;LIANG Weichao;LI Junqiao;JI Chuanliang;ZHAO Fuwei;DENG Lixin(National Engineering Research Center for Gelatin-based Traditional Chinese Medicine, Dong-E-E-Jiao Co.,Ltd., Donge 252201,China;College of Animal Science and Veterinary Medicine, Henan Agricultural University,Zhengzhou 450002,China)
出处
《中国畜牧兽医》
CAS
北大核心
2019年第2期373-379,共7页
China Animal Husbandry & Veterinary Medicine
基金
山东省农业良种工程项目(2017LZGC020)
关键词
驴
运输
应激
血液指标
donkey
transport
stress
haemotological indices
