摘要
为探明造纸废水UMIC反应器中微生物的纵向分布特性,试验应用16S rRNA基因-Illumina MiSeq高通量测序技术分析和比较了不同高度反应器中的微生物群落结构及多样性。微生物多样性分析表明,在UMIC反应器中,细菌的多样性要高于古菌,且在反应器7.5 m处微生物多样性最高。反应器的顶部拟杆菌门微生物分布较多,而绿弯菌门微生物分布较少。厚壁菌门在反应器中分布较为均匀;在UMIC反应器的1m处,Euryarchaeota丰度相对较低,而Bathyarchaeota丰度相对较高。在5 m和10 m处,Euryarchaeota占绝对优势。
In order to investigate the longitudinal distribution characteristics of microorganisms in UMIC reactor treating papermaking wastewater,the microbial community structure and diversity along the different height of reactors were analyzed and compared by using Illumina MiSeq high-throughput sequencing technology of 16S rRNA gene.The microbial diversity analysis showed that the diversity of bacteria was higher than that of archaea in the UMIC reactor,and the diversity was the highest at 7.5m of the reactor.In the top of the reactor,there were more Bacteroidetes and fewer Chloroflexi.The Firmicutes was uniformly distributed in the reactor.At about 1m of the UMIC reactor,Bathyarchaeota had a relatively higher abundance,while Euryarchaeota had a relatively lower abundance.At 5 m and 10 m of reactor,Euryarchaeota dominated.
作者
邢雅娟
谢作甫
单胜道
成忠
沈海涛
王志明
XING Ya-juan;XIE Zuo-fu;SHAN Sheng-dao;CHENG Zhong;SHEN Hai-tao;WANG Zhi-ming(.Zhejiang University of Science and Technology,School of Environmental and Natural Resources,Key Laboratory of Recycling and Eco-treatment of Waste Biomass of Zhejiang Province,Hangzhou 310023,China;Zhejiang Jingxing Paper Co Ltd,Pinghu 314214,China)
出处
《中国沼气》
2019年第1期22-26,共5页
China Biogas
基金
国家自然科学基金项目(U1609214)
浙江省重点研发计划项目(2017C03010
2015C02037)
关键词
造纸废水
厌氧颗粒污泥
高通量测序
微生物群落
papermaking wastewater
anaerobic granular sludge
MiSeq high-throughput sequencing
bacterial diversity
