摘要
目的探讨汉黄芩素对胃癌细胞增殖、迁移和侵袭的影响以及可能分子机制。方法不同浓度(20、50、100μmol/L)汉黄芩素分别作用于人胃癌SGC7901细胞24、48、72 h,空白对照不进行任何处理。采用MTT法检测细胞增殖能力;采用划痕实验观察细胞迁移能力;采用Transwell法观察细胞侵袭能力;采用流式细胞仪检测细胞周期与凋亡情况; RT-qPCR和Western blot分别对细胞基质金属蛋白酶2、9(MMP2、MMP9)、细胞间黏附分子-1(ICAM-1)以及金属蛋白酶抑制剂2(TIMP2)的mRNA和蛋白表达水平进行检测。结果 MTT实验结果显示,不同浓度的汉黄芩素抑制人胃癌SGC7901细胞呈浓度和时间依赖性;作用48 h后,汉黄芩素s期比例上升,诱导细胞凋亡(P<0. 05); Transwell实验结果显示,汉黄芩素呈浓度依赖性抑制人胃癌SGC7901细胞侵袭; RTq PCR和Western blot检测结果显示,汉黄芩素能够抑制SGC7901细胞中ICAM-1、MMP9、MMP2的mRNA和蛋白表达水平,但上调TIMP2 mRNA和蛋白表达(P<0. 05)。结论汉黄芩素能够抑制人胃癌SGC7901细胞增殖、迁移和侵袭,阻滞细胞在S周期,诱导其凋亡。
Objective To investigate the effects of wogobin on the proliferation,migration,and invasion of gastric cancer cells and their molecular mechanisms.Methods Different concentrations(20,50,and 100 mol/L)of wogobin were used to treat the hepatoma cell line SGC7901 cells for24,48,and 72 h.In the blank control,no treatment was performed.MTT assay was used to determine the cell proliferation.The migration ability of cells was observed by a scratch test.The Transwell method was used to observe cell invasiveness.Cell cycle and apoptosis were determined by flow cytometry.The mRNA and protein expression levels of cellular MMP2,MMP9,ICAM-1,and TIMP2 were determined by RT-qPCR and western blotting,respectively.Results MTT assay showed that the different concentrations of wogobin inhibited the hepatoma cell line SGC7901 in a concentration-and time-dependent manner.After 48 h,wogobin arrested the cell cycle at S-phase and induced the apoptosis in gastric cancer cells(P<0.05).Transwell assay showed that wogobin inhibited the invasion of the SGC7901 cells in a concentration-dependent manner.The RT-qPCR and western blotting analyses showed that wogobin inhibited the mRNA and protein expression of MMP9,MMP2,and ICAM-1 in SGC7901 cells,but upregulated TIMP2(P<0.05).Conclusions Wogobin inhibites the proliferation,migration,and invasion of the hepatoma cell line SGC7901 cells and induces its apoptosis.
作者
张永海
ZHANG Yonghai(Inspection Technology College of Xinyang Vocational and Technical College,Xinyang 464000,China)
出处
《中国比较医学杂志》
CAS
北大核心
2019年第2期89-95,共7页
Chinese Journal of Comparative Medicine
关键词
汉黄芩素
胃癌
细胞周期
增殖
迁移
侵袭
Wogobin
gastric cancer
cell cycle
proliferation
migration
invasion
