摘要
[Objectives] The research aimed to explore extraction method of Oenanthe benghalensis polysaccharide,and determine the optimal process of its ultrasonic extraction by orthogonal test. [Methods] Phenol-sulfuric acid method was used to determine polysaccharide's content. Based on single-factor experiment and orthogonal test,the influences of solid-liquid ratio( A),ultrasonic extraction temperature( B),ultrasonic power( C) and ultrasonic time( D) on extraction rate of O. benghalensis polysaccharide were studied,and the optimal process of ultrasonic extraction was selected. [Results] The influence order of solid-liquid ratio( A),ultrasonic extraction temperature( B),ultrasonic power( C) and ultrasonic time( D) on extraction rate of O. benghalensis polysaccharide was A > D > B > C. Via the orthogonal test,it was determined that the optimal extraction condition of O. benghalensis polysaccharide was as below: solid-liquid ratio 1∶ 25( g/mL),extraction temperature 60℃,extraction power 80 W,extraction time 40 min. Under the condition,extraction rate of O. benghalensis polysaccharide was9. 80%. [Conclusions] The process could provide the reference basis for extracting and producing O. benghalensis polysaccharides.
[Objectives] The research aimed to explore extraction method of Oenanthe benghalensis polysaccharide,and determine the optimal process of its ultrasonic extraction by orthogonal test. [Methods] Phenol-sulfuric acid method was used to determine polysaccharide's content. Based on single-factor experiment and orthogonal test,the influences of solid-liquid ratio( A),ultrasonic extraction temperature( B),ultrasonic power( C) and ultrasonic time( D) on extraction rate of O. benghalensis polysaccharide were studied,and the optimal process of ultrasonic extraction was selected. [Results] The influence order of solid-liquid ratio( A),ultrasonic extraction temperature( B),ultrasonic power( C) and ultrasonic time( D) on extraction rate of O. benghalensis polysaccharide was A > D > B > C. Via the orthogonal test,it was determined that the optimal extraction condition of O. benghalensis polysaccharide was as below: solid-liquid ratio 1∶ 25( g/mL),extraction temperature 60℃,extraction power 80 W,extraction time 40 min. Under the condition,extraction rate of O. benghalensis polysaccharide was9. 80%. [Conclusions] The process could provide the reference basis for extracting and producing O. benghalensis polysaccharides.
基金
Supported by Chinese Medicine Chemistry Construction Project in"12th Fiveyear" Key Disciplines of Chinese Medicine,State Administration of Traditional Chinese Medicine(Guozhongyiyaorenjiaofa[2012]32)
Pharmaceutical Chemistry Construction Project of Guangxi Key Discipline(Guijiaokeyan[2013]16)
Construction Program of Technology Innovation Capability Upgrading Project in Guangxi Colleges and Universities(Guijiaokeyan[2015]5)
Student's Platform for Innovation and Entrepreneurship Training Program in Youjiang Medical University for Nationalities in 2017(201710599110)
