摘要
目的探索环境浓度壬基酚(NP)暴露对大鼠焦虑行为及海马的影响。方法选取SD大鼠雌雄各20只,分为对照组(C组,玉米油)、低NP剂量(L组)、中NP剂量(M组)、高NP剂量(H组),每组10只,NP暴露剂量为0、0.02、0.20、2.00μg·kg^(-1)·d^(-1),持续90d后做高架十字迷宫和旷场实验,结束后取海马做HE染色和电镜。结果和C组比较,M、H组大鼠在开放臂停留时间缩短(P<0.05),在闭合臂停留时间缩短(P<0.05)。和C组相比,H组在中央区停留时间缩短(P<0.05),在中央区停留时间百分比降低(P<0.05),M、H组在中央区运动距离缩短(P<0.05)。HE染色观察海马CA1区发现:与C组相比,M组锥体细胞减少,组织疏松有少量空泡,H组神经元数量明显减少,细胞排列紊乱,神经元颗粒空泡变性,核溶解固缩。电镜观察海马CA1区显示:与C组对比,M组海马组织内空泡增多,神经元轻微空泡变性,线粒体肿胀,H组海马神经元间界限模糊,髓鞘溶解,胞体固缩,线粒体肿胀变性,出现空泡。结论 0.20μg·kg^(-1)·d^(-1 )NP暴露可使大鼠出现焦虑行为,2.00μg·kg^(-1)·d^(-1 )NP暴露可能通过损伤大鼠海马组织结构导致大鼠焦虑行为。
Objective To explore the effects of environmental concentration of nonylphenol(NP)exposure on anxiety behavior and hippocampus in rats.Methods Each 20 male and female SD rats were selected and divided into the control group(group C,corn oil),and high(group H)low NP dose group(group L),middle NP dose group(group M)and high NP dose group(group H),10 cases in each group.The NP exposure doses were 0,0.02,0.20,2.00μg·kg^-1·d^-1.After continuous 90 d,the elevated cross plus maze(EPM)and open field test(OFT)were taken.After end,the hippocampus was taken for conducting the HE staining and electron microscopic examinations.Results Compared with the group C,the open arm time in the group M and H was shortened,while the closed arm time was extended(P<0.05).Compared with the group C,the standing time at the central area in the group H group was decreased(P<0.05),the percentage of standing time at the central area was decreased(P<0.05)and the movement distance at the central area in the group M and H was shortened(P<0.05).The HE staining observing the hippocampus CAI area found that compared with the group C,the pyramidal cells in the group M were decreased with loose tissue and a few vacuoles,and the number of neurons in the group H was significantly decreased with disordered cell arrangement,the neuron particle vacuole denaturation,the nucleus dissolves,karyolysis and pyknosis.The electronic microscope observing the hippocampus CAI area showed that compared with the group C,the vacuoles of the hippocampus tissue in the group M were increased with slight neuron vacuolar degeneration,mitochondrial swelling,the group H showed the blurred boundary between hippocampal neurons,the cell body contraction,mitochondria swelling and degeneration and vacuoles appearance.Conclusion 0.20μg·kg^-1·d^-1 NP exposure can induce the anxiety behavior in rats,and 2.00μg·kg^-1·d^-1 NP exposure might lead to the anxiety behavior in rats by damaging the hippocampal tissue structure.
作者
严文霞
徐卫红
陈旭
杨静
罗娅
俞捷
许洁
YAN Wenxia;XU Weihong;CHEN Xu;YANG Jing;LUO Ya;YU Jie;XU Jie(School of Public Health,Zunyi Medical College,Zunyi,Guizhou 563000,China)
出处
《重庆医学》
CAS
2019年第3期382-385,共4页
Chongqing medicine
基金
国家自然科学基金资助项目(81560527
81760580)
国家大学生创新创业项目基金(201710661020)
贵州省优秀青年基金(黔科合平台人才[2017]5612)
贵州省科技厅重点项目(2018-1429
2019)
贵州省卫计委科学技术基金项目(gzwjkj2016-1-045
gzwjkj2017-1-053)
遵义市15851人才项目(2019)
遵义医学院重点学科建设经费项目(201501)
遵义医学院大学生创新基金(20163718
2018-43)
关键词
焦虑
壬基酚
大鼠
海马
anxiety
nonylphenol
rat
hippocampus
