摘要
目的探讨miR-5195-3p对Runx相关转录因子3(RUNX3)的靶向调节作用及对结直肠癌细胞凋亡的影响。方法 RT-qPCR和Western blot分别检测结直肠癌组织与癌旁组织中miR-5195-3p和RUNX3蛋白的表达。将人结肠癌细胞系SW480随机分为5组:对照组(control)、miR-5195-3p mimic组、mimic control组、miR-5195-3p inhibitor组、inhibitor control组。Wetsern blot检测RUNX3的蛋白表达;流式细胞仪检测细胞凋亡。在SW480细胞中转染RUNX3过表达质粒后检测细胞凋亡。结果与癌旁组织中相比,miR-5195-3p在结直肠癌组织中的表达显著升高,RUNX3的蛋白表达明显降低(P<0.05)。过表达miR-5195-3p显著下调了SW480细胞中RUNX3的表达,并降低细胞凋亡水平(P<0.05);抑制miR-5195-3p明显上调RUNX3的表达,并上调细胞凋亡水平(P<0.05)。此外与miR-5195-3p mimic组相比,RUNX3过表达质粒的共转染组显著降低细胞凋亡水平(P<0.05)。结论 miR-5195-3p可能作为促癌基因在结直肠癌中发挥作用,其作用与靶向调节RUNX3相关。
Objective To study the effect of miR-5195-3p on runt-related transcription factor 3 (RUNX3) and colorectal cancer cell apoptosis. Methods The mRNA expression of miR-5195-3p and protein expression of RUNX3 were detected by RT-qPCR and Western blot, respectively. SW480 cells were randomly divided into 5 groups: control group, miR-5195-3p mimic group, mimic control group, miR-5195-3p inhibitor group and inhibitor control group. The expression level of RUNX3 was measured by Western blot. Cell apoptosis was detected by flow cytometry. Results The expression of miR-5195-3p was significantly increased, while the protein expression of RUNX3 was dramatically decreased in colorectal cancer tissue as compared with non-tumor normal tissue ( P <0.05). Overexpression of miR-5195-3p significantly down-regulated RUNX3 expression and decreased cell apoptosis, while inhibition of miR-5195-3p significantly up-regulated RUNX3 expression and increased cell apoptosis of SW480 cells ( P <0.05). Additionally, co-transfection with RUNX3 overexpression vector obviously elevated cell apoptosis compared with miR-5195-3p mimic group ( P <0.05). Conclusions miR-5195-3p may act as a oncogene in colorectal cancer, and its function is related to the target regulation of RUNX3.
作者
张娟
冯燕
和水祥
ZHANG Juan;FENG Ya;HE Shui-xiang(Department of Gastroenterology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061;Department of Gastroenterology, Xinjiang Uygur Municipal People s Hospital, Urumqi 830001, China)
出处
《基础医学与临床》
CSCD
2019年第3期385-391,共7页
Basic and Clinical Medicine
