摘要
目的探索急性缺血性脑卒中(AIS)患者血浆小RNA-124(miR-124)水平变化及与病情严重程度的相关性。方法收集AIS患者40例,以40例无AIS病史体检者作为对照。MRI检测梗死体积,RT-PCR技术检测血浆miR-124表达水平。采用神经功能缺损NIHSS评分评价病情的严重程度。结果与对照组比较,AIS患者血浆miR-124水平显著降低(P <0.05)。梗死体积> 3 cm^3的AIS患者的血浆miR-124水平显著低于梗死体积<3 cm^3的AIS患者(P <0.05)。相关分析显示miR-124与梗死体积呈负相关(r=-0.473,P <0.05)。NIHSS> 5的AIS患者的血浆miR-124水平显著低于NIHSS≤5的AIS患者(P <0.05)。NIHSS评分与miR-124水平呈负相关(r=-0.567,P <0.05)。结论 AIS患者血浆miR-124水平显著降低,其水平与脑梗死体积及NIHSS评分呈负相关。
Objective To investigate the expression and clinical significance of plasma miR-124 in acute ischemic stroke(AIS). Methods Forty patients with AIS were collected and 40 volunteers without history of AIS were set as control. Infarction volume was detected by MRI;plasma miR-124 expression level was measured by RTPCR technique and neural function was evaluated by NIHSS scores. Results Compared with that in the control group,plasma miR-124 level in AIS group was significantly reduced(P < 0.05). Plasma miR-124 level in AIS patients with infarction volume greater than 3 cm3 was significantly lower than that of AIS patients with infarction volume less than 3 cm3(P < 0.05). Correlation analysis showed a negative correlation between miR-124 and infarction volume(r =-0.473,P < 0.05). Plasma miR-124 level in AIS with NIHSS score higher than 5 was significantly lower than that of AIS patients with NIHSS lower than 5(P < 0.05). NIHSS score negatively correlated with the miR-124 level of AIS patients(r =-0.567,P < 0.05). Conclusion The plasma miR-124 is significantly reduced in patients with AIS,and negatively correlated with the cerebral infarction volume and NIHSS score.
作者
谢尊椿
刘彬
周美鸿
陈永康
XIE Zunchun;LIU Bin;ZHOU Meihong;CHEN Yongkang(Department of Neurology,the First Affiliated Hospital of Nanchang University,Nanchang 330006,China)
出处
《实用医学杂志》
CAS
北大核心
2019年第3期343-345,共3页
The Journal of Practical Medicine
基金
国家自然科学基金项目(编号:81302892)
