摘要
目的探讨程序性死亡配体-1(programmed death ligand-1, PD-L1)和粘蛋白1(mucin 1, MUC1)在胃癌组织中的表达与临床病理因素间的关系以及两者间的相互关系。方法应用免疫组织化学S-P方法检测PD-L1和MUC1在120例胃癌组织和40例距癌灶边缘5cm以上的正常胃粘膜组织中的表达。结果在正常胃粘膜组织中未见PD-L1阳性免疫反应,在胃癌组织中PD-L1阳性表达率为32.5%(39/120);MUC1在正常胃粘膜组织中的阳性表达率为100%,在胃癌组织中的阳性表达率是65%(78/120)。在胃癌组织中,PD-L1和MUC1表达与肿瘤大小、肿瘤浸润深度及淋巴结转移有关,与患者年龄、性别、肿瘤分化程度及TNM分期无关;PD-L1和MUC1在胃癌组织中的表达呈正相关。结论 PD-L1和MUC1在胃癌组织中的生物学行为以及两者的相关性提示联合检测更有意义。
Objective To investigate the relationship between expression and clinicopathological factors of programmed death ligand-1 (PD-L1) and mucin 1 (MUC1) as well as their correlation in gastric carcinoma. Methods Immunohistochemical staining (S-P method) for PD-L1 and MUC1 protein was performed in 120 cases of gastric carcinoma tissue and 40 cases of normal gastric mucosa tissue at the margin of 5 or more cm from the edge of cancer tissue. Results No PD-L1 positive immunoreactivity was observed in the normal gastric mucosa and the PD-L1 positive rate was 32.5%(39/120) in the gastric carcinoma tissue;MUC1 positive rate was 100% in the normal gastric mucosa and 65%( 78/120) in the gastric carcinoma mucoca. The expression of PD-L1 and MUC1 was correlated with tumor size, depth of tumor invasion and lymph node metastasis, but not correlated with age, gender, tumor differentiation and TNM stage. PD-L1 was positively correlated with MUC1 in the expression level. Conclusion The biological behavior of PD-L1 and MUC1 proteins in gastric cancer tissues and their correlation suggest that the combined detection for both PD-L1 and MUC1 proteins is more meaningful.
作者
陆娜娜
Lu Nana(Department of pathology, the Binhu Hospital of Hefei, Hefei 230601)
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2018年第6期553-558,共6页
Chinese Journal of Histochemistry and Cytochemistry
