摘要
采用AB-8大孔树脂和硅胶柱层析技术纯化并分离黑树莓花色苷,分别通过DPPH自由基、ABTS自由基和羟自由基清除试验评价花色苷组分的抗氧化活性。结果表明:经过大孔树脂纯化后的花色苷的纯度从17.18%提高到85.21%,得率为0.60%;硅胶柱层析参数为洗脱液配比为正丁醇∶乙酸∶水的体积比为4∶2∶0.5,流速为0.5 mL/min,树莓花色苷粗品与硅胶的质量比为1∶550;得到黑树莓花色苷产物组分分别为BRA1和BRA2,纯度分别为94.53%和92.14%。组分BRA1的抗氧化活性优于组分BRA2,两者均呈现出良好的抗氧化活性。
The black raspberry anthocyanins were purified and separated by AB-8 macroporous resin and silica gel column chromatography, and the antioxidant activity of anthocyanin components was evaluated by DPPH free radical, ABTS free radical and hydroxyl radical scavenging experiments. The results showed that the purity of anthocyanins increased from 17.18% to 85.21% after macroporous resin purification, and the yield was 0.60 %. The separation parameters of silica gel column chromatography were as follows: The ratio of eluent was n-butanol ∶ acetic acid ∶ water =4 ∶ 2 ∶ 0.5, The flow rate was 0.5 mL/min, black raspberry anthocyanin crude products ∶ silica gel=1 ∶ 550;The anthocyanins components BRA1 and BRA2 were collected, the purities of them were 94.53 % and 92.14 %, respectively. The antioxidant activity of the component BRA1 was higher than that of the component BRA2, both of them showed good antioxidant activity.
作者
王雅琼
王远辉
张缤聿
马恒宇
WANG Ya-qiong;WANG Yuan-hui;ZHANG Bin-yu;MA Heng-yu(College of Food Science and Technology,College of International Education,Henan University ofTechnology,Zhengzhou 450001,Henan,China)
出处
《粮食与油脂》
北大核心
2019年第3期80-85,共6页
Cereals & Oils
基金
河南工业大学高层次人才基金(2015BS009)
河南工业大学"科教融合"项目(26400030)
关键词
薄层色谱
高效液相色谱
大孔树脂
硅胶柱层析
自由基
thin-layer chromatography
high performance liquid chromatography
macroporous resin
silica gel column chromatography
free radical
