摘要
为培养优质的铜绿假单胞菌Pseudomonas aeruginosa菌液,通过单因素试验对草鱼铜绿假单胞菌灭活疫苗发酵培养基的氮源、碳源和磷酸盐成分进行了筛选,采用正交试验法对培养基各主要成分的用量进行了优化组合,并经过验证试验绘制出了铜绿假单胞菌JP802在优化培养基条件下的5 L发酵罐生长曲线。结果表明:草鱼赤皮病铜绿假单胞菌JP802发酵培养基中最佳氮源为蛋白胨+牛肉膏+酵母膏,最佳碳源为葡萄糖,最佳磷酸盐为磷酸氢二钾;确立了培养基的优化配方为蛋白胨10 g/L、牛肉膏5.0 g/L、酵母膏2.5 g/L、葡萄糖5.0 g/L、磷酸氢二钾0.75 g/L、氯化钠5.0 g/L,JP802菌株在此培养基中发酵14 h菌体浓度达到最大(OD_(600 nm)值为6.44)。研究表明,通过对发酵培养基的优化,可以获得更高产量的铜绿假单胞菌JP802发酵菌液。
The nitrogen source, carbon source and phosphate were screened in culture medium of Pseudomonas aeruginosa inactivated vaccine in a single-factor test, and different ingredients of the medium were optimized in an orthogonal experiment, with a verification test of growth of the bacterium in a 5 L fermentor. It was found that the best fermentation of Pseudomonas aeruginosa in grass carp Ctenopharyngodon idellus with red skin disease was observed under the conditions of peptone+beef extract+yeast extract as nitrogen source, glucose as carbon source, K 2 PHO 4 as phosphate source, at a dose of peptone 10 g/L, beef extract 5.0 g/L, yeast extract 2.5 g/L, glucose 5.0 g/L , K 2HPO 4 0.75 g/L, and NaCl 5.0 g/L. The fermentation kinetics analysis revealed that the stain had the maximal cell density at 6.44 of OD 600 nm in 14 h culture, indicating that a high yield of Pseudomonas aeruginosa JP802 fermentation broth is obtained by the optimized fermentation medium.
作者
孙承文
赖迎迢
任小波
江小燕
巩华
黄志斌
陶家发
SUN Cheng-wen;LAI Ying-tiao;REN Xiao-bo;JIANG Xiao-yan;GONG Hua;HUANG Zhi-bin;TAO Jia-fa(Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China;College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2019年第1期15-20,共6页
Journal of Dalian Ocean University
基金
中国水产科学研究院基本科研业务费专项(2016HY-ZD0502)
广东省科技计划项目(2016B020234003)
关键词
草鱼
铜绿假单胞菌
发酵培养基
优化
Ctenopharyngodon idellus
Pseudomonas aeruginosa
fermentation medium
optimization
