摘要
目的研究雷公藤多苷类脂质体贴剂的透皮机制,比较雷公藤多苷片及雷公藤多苷类脂质体贴剂的抗炎作用。方法采用Franz扩散池法,进行体外透皮释放,用多种释药模型对释药曲线进行拟合。将50只ICR小鼠随机分为空白对照组、模型组、雷公藤多苷片组(9.1 mg·kg^(-1))及雷公藤多苷类脂质体贴剂高、低剂量组(9.1和4.5 mg·kg^(-1)),通过二甲苯致耳肿胀建立小鼠急性炎症模型,观察小鼠耳肿胀度及肿胀抑制率,检测小鼠血清中白细胞介素-1(IL-1)和肿瘤坏死因子-α(TNF-α)的含量及脾、胸腺的脏器指数。结果雷公藤多苷类脂质体贴剂的透皮释放曲线与Higuchi模型拟合最好,能达到缓释作用。与空白对照组比较,模型组小鼠耳肿胀度、胸腺指数升高,血清中IL-1和TNF-α含量显著增加(P<0.05);与模型组比较,雷公藤多苷片组和雷公藤多苷类脂质体贴剂高、低剂量组均能明显抑制小鼠耳肿胀,显著降低小鼠血清中IL-1和TNF-α含量(P<0.05),雷公藤多苷类脂质体贴剂高、低剂量组胸腺指数均显著下降(P<0.05);与雷公藤多苷片组比较,雷公藤多苷类脂质体贴剂高剂量组能显著抑制小鼠耳肿胀并减少血清中IL-1和TNF-α的含量,差异有统计学意义(P<0.05)。结论雷公藤多苷类脂质体贴剂比雷公藤多苷片表现出明显的抗炎作用。
Objective To study the transdermal mechanism of liposome patches loaded with Tripterygium wilfordii polyglycosides and compare the anti-inflammatory effects of Tripterygium glycosides patches and Tripterygium glycosides tablets. Methods Franz diffusion cell method was used to carry out the transdermal release in vitro ,and the drug release curve was fitted by multiple drug release model.50 ICR mice were randomly divided into control group,model group,Tripterygium Tablets group (9.1 mg·kg^-1 ),high and low dose group of patches of liposomes loaded with Tripterygium glycosides (9.1 and 4.5 mg·kg^-1 ).The ear swelling was induced by xylene to establish the mouse model.The acute inflammation,mouse ear swelling and swelling inhibition rate,the interleukin 1 (IL-1) and tumor necrosis factor alpha (TNF-α) contents in serum,and organ index of spleen and thymus were observed or detected.Results The transdermal delivery curve of Tripterygium wilfordii glycosides patches was fitted to the Higuchi model well.Compared with the blank control group,the ear swelling as well as thymus index of mice in model group increased,and the contents of IL-1 and TNF-α in serum increased significantly ( P <0.05).Compared with the negative control,the ear swelling of mice of Tripterygium glycosides high as well as low dose group of patches loaded with Tripterygium glycosides were inhibited,the contents of IL-1 and TNF-α in serum of mice decreased significantly ( P <0.05),thymus index of mice of high and low dose group of patches of liposomes loaded with Tripterygium glycosides decreased significantly ( P <0.05).Compared with the model group,the ear swelling of mice in high and low dose group of patches of liposomes loaded with Tripterygium glycosides was inhibited,and the contents of IL-1 and TNF-α in serum decreased significantly ( P <0.05).Compared with the Tripterygium Tablets group,the ear swelling of mice in high dose group of patches of liposomes loaded with Tripterygium glycosides was inhibited,and the contents of IL-1 and TNF-α in serum decreased significantly ( P <0.05).Conclusion Patches of liposomes loaded with Tripterygium glycosides through diffusion showed significant anti-inflammatory effect.
作者
郭毅
孙敬蒙
贾学娇
王丹
张炜煜
GUO Yi;SUN Jingmeng;JIA Xuejiao;WANG Dan;ZHANG Weiyu(Changchun University of Chinese Medicine,Changchun 130117,China;The First Hospital of Jilin University,Changchun 130000,China)
出处
《西北药学杂志》
CAS
2019年第2期209-212,共4页
Northwest Pharmaceutical Journal
关键词
雷公藤多苷
类脂质体
贴剂
抗炎作用
Tripterygium glycosides
liposomes
transdermal patch
anti-inflammatory effect
