摘要
【目的】DNA甲基化是高等植物中普遍存在的一种表观修饰形式,其在调节基因表达、维持基因组稳定以及调控植物生长发育等方面发挥重要作用。本研究拟对基因组甲基化如何影响水稻发育进行解析。【方法】利用DNA甲基化抑制剂5-氮脱氧胞苷(AZA, 5-Aza-2′-deoxycytidine)处理水稻幼苗,研究DNA甲基化抑制剂对水稻幼苗生长发育及相关基因表达的影响。【结果】AZA处理后水稻基因组甲基化水平下降、植株发育迟缓,但种子萌发并不受AZA处理的影响;DNA和组蛋白表观修饰相关基因的表达受到AZA处理抑制。此外,防卫反应和光合通路相关基因表达也受到AZA处理的影响,暗示DNA甲基化在这些基因的表达调控中可能发挥作用。【结论】这些结果表明AZA是一种有效的DNA甲基化抑制剂,AZA处理可以破坏水稻基因组甲基化水平的正常状态,从而影响水稻的正常发育。
【Objective】 DNA methylation is an epigenetic modification broadly existed in higher plants. DNA methylation plays an important role in regulating gene expression, maintaining the genomic stability and modulating plant development. Here, we used a DNA methylation inhibitor, 5-Aza-2′-deoxycytidine (AZA), to study how the DNA methylation status influences rice development.【Method】We applied AZA to the rice seedlings to explore its influence on seedling development.【Results】AZA-treatment resulted in overall hypomethylation of the rice genome. AZA-treated plants were strikingly shorter than the control, indicating AZA treatment disturbed normal development of the plants. However, the seed germination was not significantly affected. Expression of several key genes involved in DNA methylation could be repressed by AZA treatment. In addition, some genes involving defense and photosynthesis pathways were also down-regulated.【Conclusion】Our findings confirm that AZA is a potent DNA methylation inhibitor and suggest that to maintain proper DNA methylation status is important for rice development.
作者
邓卉
鄂志国
牛百晓
王磊
陈忱
DENG Hui;E Zhiguo;NIU Baixiao;WANG Lei;CHEN Chen(Jiangsu Key Laboratory of Crop Genetics and Physiology / Key Laboratory of Plant Functional Genomics of the Ministry of Education / Jiangsu KeyLaboratory of Crop Genomics and Molecular Breeding / Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,YangzhouUniversity,Yangzhou 225009,China;State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 311401,China)
出处
《中国水稻科学》
CAS
CSCD
北大核心
2019年第2期108-117,共10页
Chinese Journal of Rice Science
基金
国家自然科学基金资助项目(31571623
31771744)
植物功能基因组学教育部重点实验室开放课题资助项目(ML201805)
