摘要
目的探讨抗β_2糖蛋白Ⅰ抗体、膜糖蛋白GPⅠb、抗链球菌溶血素O检测在类风湿关节炎急性阶段的诊断价值。方法收集67例类风湿关节炎急性阶段患者为病例组,选取院内体检健康者50例为对照组。采用酶联免疫吸附试验检测抗β_2糖蛋白Ⅰ抗体,流式细胞术检测血小板膜糖蛋白GPⅠb的表达,免疫比浊法检测抗链球菌溶血素O的水平。采用Pearson分析进行相关分析,利用受试者工作特征曲线(ROC曲线)分析抗β_2糖蛋白Ⅰ抗体、血小板膜糖蛋白GPⅠb、抗链球菌溶血素O诊断类风湿关节炎急性阶段的曲线下面积(AUC)。结果病例组抗β_2糖蛋白Ⅰ抗体、膜糖蛋白GPⅠb、抗链球菌溶血素O水平均高于对照组,差异有统计学意义(P<0.01)。Pearson相关性分析中,抗β_2糖蛋白Ⅰ抗体、膜糖蛋白GPⅠb、抗链球菌溶血素O与类风湿关节炎急性阶段呈正相关(r=0.514、0.620、0.710)。ROC曲线分析显示,抗β_2糖蛋白Ⅰ抗体、膜糖蛋白GPⅠb、抗链球菌溶血素O的AUC分别为0.82、0.82、0.91,最佳诊断界值分别为21.84RU/mL、14.79%、51.32IU/mL。结论抗β_2糖蛋白Ⅰ抗体、膜糖蛋白GPⅠb、抗链球菌溶血素O检测对类风湿关节炎急性阶段具有一定的诊断价值,可作为类风湿关节炎急性阶段的辅助诊断指标。
Objective To investigate the diagnostic value of anti-β2 glycoprotein Ⅰ antibody,membrane glycoprotein GPⅠb and anti-streptolysin O in the acute stage of rheumatoid arthritis.Methods Sixty-seven patients with acute rheumatoid arthritis were selected as case group,and 50 healthy persons were selected as control group.Anti-β2 glycoprotein Ⅰ antibody was detected by ELISA,platelet membrane glycoprotein GPⅠb expression was detected by flow cytometry,and anti-streptolysin O was detected by immunoturbidimetry.Pearson analysis was used for correlation analysis.The area under curve(AUC)of anti-β2 glycoprotein Ⅰ antibody and platelet membrane glycoprotein GPⅠb and anti-streptolysin O in the diagnosis of acute rheumatoid arthritis was analyzed by ROC curve.Results The anti-β2 glycoprotein Ⅰ antibody,membrane glycoprotein GPⅠb and anti-streptolysin O levels in the case group were significantly higher than those in the control group(P<0.01).Pearson correlation analysis showed that anti-β2 sugar protein Ⅰ antibody,membrane glycoprotein GPⅠb,anti-streptolysin O were positively correlated with the acute phase of rheumatoid arthritis(r=0.514,0.620,0.710);ROC curve analysis showed that the AUC of anti-β2 glycoprotein Ⅰ antibody,membrane glycoprotein GPⅠb and anti-streptolysin O were 0.82,0.82,0.91,respectively,and the best diagnostic cutoff values were 21.84 RU/mL,14.79%,51.32 IU/mL,respectively.Conclusion The anti-β2 glycoprotein Ⅰ antibody,membrane glycoprotein GPⅠb and anti-streptolysin O detection have certain diagnostic value in the acute stage of rheumatoid arthritis,and can be used as an auxiliary diagnostic index for the acute stage of rheumatoid arthritis.
作者
高明
杨晓英
毛晓莉
GAO Ming;YANG Xiaoying;MAO Xiaoli(Department of Blood Transfusion,South Hospital,Sixth People′s Hospital Affiliated to Shanghai Jiaotong University,Shanghai 201499,China;Department of Blood Transfusion,Fengxian District Central Hospital,Shanghai 201499,China)
出处
《国际检验医学杂志》
CAS
2019年第5期575-578,共4页
International Journal of Laboratory Medicine
基金
国家自然科学基金资助项目(81572034)
上海市卫生和计划生育委员会青年基金(20164Y0156)
关键词
抗β2糖蛋白Ⅰ抗体
膜糖蛋白
抗链球菌溶血素O
类风湿关节炎急性阶段
anti-β2 glycoprotein Ⅰ antibody
membrane glycoprotein
anti-streptococcal hemolysin O
acute stage of rheumatoid arthritis
