摘要
本论文对茯苓液体菌种培养条件和栽培基质进行优化。利用摇床在25℃恒温条件下培养,通过单因素和响应面优化实验对茯苓液体发酵条件进行分析,从茯苓液体发酵中摇瓶转速、接种量、发酵时间等因素对茯苓液体发酵的最优条件进行了探讨,结果表明:以150 m L液体培养基进行培养,摇瓶转速为177 r/min,接种量为6.62 m L,发酵时间为8 d,茯苓液体发酵的条件最佳;茯苓最佳的栽培基质配方为:32%草灰、26%玉米芯、18%米糠、16%麸皮、6%豆粕、1%石膏、1%石灰,生物学效率为64%。
In this paper, the conditions of culturing Poria cocos liquid strain and culturing matrix were optimized using the table in 25 ℃ conditions. The fermentation conditions of Poria cocos were analyzed by single factor and response surface optimization experiment.According to the experimental results, the optimal conditions were obtained as follows: culture on 150 mL liquid medium, rotation speed of shaking flask was 177 r/min, and inoculation amount was 6.62 mL, and fermentation time was 8 days. Highest biological efficiency value was 64%. Using a cultivation substrate consisting of 32% straw ash, 26% corn cobs,18% rice hulls, 6% soybean powder, 16% wheat bran and 1% gypsum and 1% lime.
作者
李作美
黄奇
LI Zuomei;HUANG Qi(School of Food and Biology Engineering,Bengbu University,Bengbu Anhui 233000,China)
出处
《阜阳师范学院学报（自然科学版）》
2019年第1期30-35,共6页
Journal of Fuyang Normal University(Natural Science)
基金
安徽省质量工程项目(2016ckjh118)
安徽高校自然科学研究项目(KJ2018A0570)资助
关键词
响应面
茯苓
液体发酵
基质优化
response surface
poria cocos
liquid fermentation
optimization of cultivation substrate
