摘要
针对微藻生物柴油发展的迫切需求,研究微藻异养培养产油以及通过盐胁迫方法提高微藻的油脂累积量。实验过程中首先从污水处理厂、天然水体及养殖厂废水等环境中分离纯化了14株微藻藻株,通过对比分析它们的光自养生长量和光异养生长量,确认其中4株具有异养生长能力,并从中优选了1株异养生长能力最优良的小球藻作为本实验的对象进行进一步研究。实验过程中对比分析了葡萄糖、甲醇、乙醇、无水乙酸钠、六水丁二酸钠等5种有机碳源对该藻株异养生产的影响,结果表明葡萄糖作为异养生长的有机碳源时微藻获得了最大的生物生长量(1.63 g/L)和油脂产量(0.57 g/L),因此优选了葡萄糖作为该藻株异养生长的理想有机碳源。进一步研究了盐胁迫下微藻异养生长及油脂累积的特性,结果表明在异养培养基盐度为0.4%时,微藻生长及油脂累积受盐度的影响较小,当盐度上升至0.8%、1.2%、1.6%和2.0%时,微藻的油脂含量随着盐度的上升而上升,在盐度为2.0%时微藻的油脂含量相比于生长在不添加NaCl培养基中的微藻提升约60%左右,达到47.75%的较高水平。但由于盐度胁迫同时也减少了微藻生长量,因此盐度胁迫下微藻的油脂产量没能得到提升,需要后续的研究探索。
This study investigated the lipid production of microalgae in heterotrophic culture and the increase of algal lipid accumulation through salt stress. In the process of experiment, 14 strains of algae were isolated and purified from the wastewater treatment plant, natural water body and aquaculture wastewater. By comparing and analyzing the light autotrophic growth and light heterotrophic growth of these microalgae strains,4 of them were proved to have the ability of heterotrophic growth. In addition, one Chlorella vulgaris with the best heterotrophic growth ability was selected as the object of this experiment. The effects of five organic carbon sources including glucose, methanol, ethanol, sodium acetate and sodium succinate dibasic hexahydrate on the heterotrophic growth of the Chlorella were compared. The results showed that the maximum biomass(1.63 g/L) and lipid production(0.57 g/L) were obtained when the microalgae were cultured in the medium containing glucose. In this experiment, the characteristics of algal heterotrophic growth and lipid accumulation under salt stress were further studied. The results showed that when the salinity of heterotrophic medium was 0.4%,the growth of microalgae and the accumulation of oil were less affected by salinity. When salinity of the culture medium increased to 0.8%, 1.2%, 1.6%, and 2%, the lipid content of microalgae increased with the increase of salinity. When the salinity of the culture medium was 2%, the oil content of microalgae reached a higher level of 47.75%, which was about 60% higher than that of the microalgae growing in the medium without NaCl.However, salt stress also reduced the growth of microalgae. Therefore, the yield of algal lipid under salt stress could not be improved, so more researches were needed to further improve the algal lipid production.
作者
崔伟
徐晶萍
芦文城
苏晓晨
高锋
鲍静姣
郭远明
CUI Wei;XU Jing-ping;LU Wen-cheng(School of Ocean Science and Technology of Zhejiang Ocean University, Zhoushan 316022, China)
出处
《浙江海洋大学学报（自然科学版）》
CAS
北大核心
2018年第6期505-509,共5页
Journal of Zhejiang Ocean University：Natural Science
基金
浙江省科技厅项目(2018F10012)
浙江省海洋与渔业局项目([2013]111).
关键词
微藻
异养培养
盐胁迫
油脂累积
microalgae
heterotrophic culture
salt stress
lipid accumulation
