摘要
目的·观察温敏性羟丁基几丁糖(hydroxybutyl chitosan,HBC)水凝胶预防新西兰大白兔宫腔粘连的效果。方法·将18只雌性新西兰大白兔随机分为3组,即正常对照组、模型组、HBC组,每组6只。正常对照组行假手术;模型组、HBC组采用机械和感染双重损伤法构建宫腔粘连动物模型;HBC组建模后立即向宫腔内注射2 mL 1.5%的温敏性HBC水凝胶。每组分别于术后1、2、4周各取2只新西兰大白兔,收集双侧子宫组织,苏木精-伊红染色观察子宫内膜形态及腺体数量;Masson染色检测子宫内膜纤维化程度。结果·与正常对照组相比,模型组与HBC组术后1周子宫内膜柱状上皮逐渐消失,纤维化面积比明显增加,腺体数量减少。2周后,模型组低倍镜下可见宫腔内粘连形成,纤维化面积比进一步增加,腺体数量进一步减少;而HBC组宫腔内未见水凝胶残余,纤维化面积比下降,腺体数量增加。4周后,HBC组子宫内膜柱状上皮恢复,纤维面积比与腺体数量均恢复至正常水平。结论·温敏性HBC水凝胶可以有效预防新西兰大白兔宫腔粘连形成。
Objective · To observe the effect of thermosensitive hydroxybutyl chitosan (HBC) hydrogel in the prevention of intrauterine adhesion in New Zealand white rabbits. Methods · Eighteen female New Zealand rabbits were randomly divided into 3 groups, i.e., normal control group, model group and HBC group. Normal control group underwent sham operation. The models of intrauterine adhesion were constructed by both mechanical damage and infection in model group and HBC group. In HBC group, 2 mL 1.5% thermosensitive HBC hydrogel was injected into the uterine cavity immediately after injury. Two rabbits were killed in each group 1 week, 2 weeks and 4 weeks after operation, respectively. The bilateral uterine tissues were collected. The endometrial morphology and quantity of glands were observed by hematoxylin-eosin staining. The area of fibrosis in endometrium was measured by Masson staining. Results · One week after operation, compared with normal control group, the columnar epithelial cells of endometrium gradually disappeared and the ratio of endometrial fibrosis area increased significantly in the other two groups. The number of glands also decreased. After 2 weeks, intrauterine adhesion was observed in model group, and the ratio of endometrial fibrosis area continued increasing, and the number of glands decreased further. However, in HBC group, there was no residual hydrogel in the uterine cavity, and the ratio of endometrial fibrosis area decreased and the number of glands increased. After 4 weeks, there was a recovery of columnar epithelium cells, the ratio of endometrial fibrosis area, and the number of glands in HBC group, which returned to the normal level. Conclusion · Thermosensitive HBC hydrogel can effectively prevent intrauterine adhesion in New Zealand white rabbits.
作者
吴若愚
曹忆梦
吴氢凯
邱雨
黄程胜
薛卓维
WU Ruo-yu;CAO Yi-meng;WU Qing-kai;QIU Yu;HUANG Cheng-sheng;XUE Zhuo-wei(Department of Obstetrics and Gynecology,Shanghai Sixth People's Hospital,Shanghai Jiao Tong University,Shanghai 200233,China)
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2019年第2期131-135,共5页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金(81550008
81771523)
上海市科学技术委员会产学研医合作项目(12DZ1940406)~~
关键词
温敏性羟丁基几丁糖水凝胶
宫腔粘连
新西兰大白兔
thermosensitive hydroxybutyl chitosan hydrogel
intrauterine adhesion
New Zealand white rabbit
