摘要
枯草芽胞杆菌作为革兰阳性模式菌株是基础研究和工业应用的常用宿主细胞。介绍了枯草芽胞杆菌中蛋白合成和分泌过程中的重要步骤及重要调控位点。在枯草芽胞杆菌蛋白表达及分泌系统中,可以针对目标基因在体内的转录、翻译、折叠、转运和菌株改造等方面对表达分泌系统进行优化改良,针对不同的目标蛋白,可进行不同优化模块的组装和拼搭,以达到针对目标蛋白产物定制化地提高产量和分泌量的目的。在未来,随着基因编辑和合成生物技术的发展,菌株改良策略的不断优化,枯草芽胞杆菌将会在工业生产蛋白质制品领域发挥更大的应用价值。
Bacillus subtilis is a common gram-positive model strain and a host cell with great industrial application potential. In this review, the important steps and regulatory sites in protein synthesis and secretion in Bacillus subtilis were introduced. In Bacillus subtilis protein expression and secretion system, the modification of protein expression and secretion system can aim at the transcription of target genes, protein translation, folding, transport and strain optimization improvement. According to different target proteins, the different optimization modules can be assembled, for achieving customization of improving target proteins production, increasing the amount of protein secretion. In the future, accompanying with the further developing of gene editing, synthetic biotechnology and continuous optimization of strain improvement strategy, Bacillus subtilis will play a more important role in protein production.
作者
张大伟
康倩
ZHANG Da-wei;KANG Qian(CAS key laboratory of system microbial biotechnology,Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308;University of Chinese Academy of Sciences,Beijing 100049)
出处
《微生物学杂志》
CAS
CSCD
2019年第1期1-10,共10页
Journal of Microbiology
基金
国家重点研发计划项目(2018YFD0901001)
天津市杰出青年科学基金项目(17JCJQJC45300)
天津市自然科学基金项目(16JCYBJC23500)
关键词
枯草芽胞杆菌
蛋白质表达和分泌
微生物应用
表达分泌系统调控
菌株改造策略
Bacillus subtilis
protein expression and secretion
microbial application
regulation of expression and secretion system
strain modification strategy
