摘要
目的:观察益气化瘀散结方对胃癌SGC-7901细胞增殖凋亡的影响,并通过益气化瘀散结方干预后凋亡相关蛋白Survivin、Bcl-2的表达变化探讨其相关作用机制。方法:体外培养SGC-7901细胞,采用益气化瘀散结方含药血清干预,细胞分为空白血清组及5%、10%、20%益气化瘀散结方含药血清组,药物干预后,CCK_8法检测细胞增殖情况,流式细胞术检测细胞凋亡,Western-blot、Real-time PCR技术检测Survivin、Bcl-2蛋白及mRNA的表达变化。结果:5%、10%、20%益气化瘀散结方含药血清组SGC-7901细胞的增殖明显下降,与空白血清组比较,10%、20%浓度组差异显著(P<0.05),48 h为实验最佳干预时间点;5%、10%、20%益气化瘀散结方含药血清均能不同程度促进SGC-7901细胞凋亡,尤以10%、20%含药血清组差异显著(P<0.05);10%、20%浓度益气化瘀散结方含药血清组Survivin、Bcl-2蛋白及mRNA表达水平下降,与空白血清组比较差异显著(P<0.05)。结论:益气化瘀散结方能抑制胃癌SGC-7901细胞增殖、促进胃癌SGC-7901细胞凋亡,其机制可能与降低凋亡抑制蛋白Survivin、Bcl-2的表达相关。
Objective:To observe the effect of Yiqi Huayu Sanjie decoction on proliferation and apoptosis of cancer cell Line of SGC-7901,and explore the mechanism by expression changes of Survivin and Bcl-2 after intervention with Yiqi Huayu Sanjie decoction. Methods:SGC-7901 cells were cultured in vitro and treated with drug-containing serum of Yiqi Huayu Sanjie decoction,cells were devided into four groups:blank serum group,5%、10%、20% of drug-containing serum of Yiqi Huayu Sanjie decoction groups,the proliferation rate was detected by CCK 8,the cell apoptosis was detected by flow cytometry,the protein and mRNA expression of Survivin and Bcl-2 were detected by Western-blot and Real-time PCR. Results:Compared with the blank serum group,each concentration of Yiqi Huayu Sanjie decoction could inhibit the proliferation of SGC-7901 cells,in which 10% and 20% concentration significant especially ( P <0.05),the most obvious time was 48 h;Compared with the blank serum group,each concentration of Yiqi Huayu Sanjie decoction could promote apoptosis of SGC-7901 cells,especially the 10% and 20% concentration groups ( P <0.05);Compared with the blank serum group,the expression of Survivin and Bcl-2 protein and mRNA in serum of different concentration of Yiqi Huayu Sanjie decoction were significantly decreased( P <0.05). Conclusion:Yiqi Huayu Sanjie decoction can inhibit the proliferation and promote apoptosis of gastric cancer SGC-7901 cells,the mechanism of which may be related to the reduction of the expression of apoptosis-inhibiting protein Survivin and Bcl-2.
作者
裴俊文
孙太振
蒋立峰
PEI Junwen;SUN Taizhen;JIANG Lifeng(The Affiliated Cancer Hospital of Zhengzhou University(Henan Cancer Hospital),Zhengzhou450008)
出处
《陕西中医》
2019年第4期411-414,共4页
Shaanxi Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(U1704181)
河南省科技攻关计划项目(082102310028)
