摘要
旨在通过对FGF21、受体FGFR1和FGFR2在小鼠毛囊第1生长周期中的定位和表达情况的研究,以探索FGF21、受体FGFR1和FGFR2在毛囊发育过程中的作用。选取1、3、5、8、12、17、21和23日龄小鼠背部皮肤,采用免疫组织化学技术,实时荧光定量PCR和蛋白免疫印迹技术检测FGF21、FGFR1和FGFR2 mRNA及蛋白表达。结果显示:在小鼠毛囊第1生长周期中,FGF21主要表达于毛囊毛乳头、毛基质、内外根鞘以及毛囊周围的结缔组织中;FGFR1在毛囊各部均有表达,且在退化期(12～17日龄)和静止期(18～21日龄)主要表达于内外根鞘中;FGFR2广泛表达于毛囊各部。1、3、5、8、12、17日龄FGF 21 mRNA表达量极显著低于23日龄(P<0.01),21日龄的表达量低于23日龄,差异不显著(P>0.05);1～5日龄和23日龄FGF21蛋白表达量较高。1、3、5、8、12、21、23日龄FGFR1 mRNA表达量极显著低于17日龄(P<0.01);FGFR1蛋白表达量从12日龄开始上升,在17日龄时达到最高,之后又呈下降趋势。1、5、8、12、21和23日龄FGFR2 mRNA表达量极显著低于17日龄(P<0.01),在3日龄的表达量低于17日龄,差异不显著(P>0.05);FGFR2蛋白在1～17日龄都有较高表达。综上表明,在第1个毛囊生长周期中,FGF21对参与毛囊形成的成纤维细胞的增殖和分化具有重要作用,诱导毛囊由生长期进入退化期。FGFR1对内外根鞘细胞的增殖、分化有着明显作用,诱导毛囊由退化期进入静止期。FGFR2对毛囊内细胞增殖、分化具有重要作用,诱导毛囊由生长期和退化期进入静止期。
To explore the role of FGF21 and receptors FGFR1, FGFR 2 in hair follicle development, we researched the localization and expression of FGF21 and receptors FGFR1, FGFR2 in the first hair follicle growth cycle of mice. The immunohistochemistry, real-time PCR and Western blot were used to study the expression of FGF21 and receptors FGFR1, FGFR2 mRNA and proteins in mice back skin on 1, 3, 5, 8, 12, 17, 21, 23 days old. The results showed that during the first hair follicle growth cycle of mice, FGF21 was mainly expressed in dermal papilla, matrix cells, inner and outer root sheaths and connective tissue around hair follicles;FGFR1 was located in various structures of hair follicle, and abundantly expressed in the inner and outer root sheaths in the catagen(12-17 days old) and telogen(18-21 days old);FGFR2 was abundantly located in various structures of hair follicle. The expression levels of FGF 21 mRNA at 1, 3, 5, 8, 12 and 17 days old were significantly lower than at 23 days old( P <0.01), and the expression at 21 days old was lower than at 23 days old, the difference was not significant( P >0.05);The expression levels of FGF21 protein were higher at 1-5 days and 23 days old. The expression levels of FGFR 1 mRNA at 1, 3, 5, 8, 12, 21 and 23 days old were significantly lower than at 17 days old( P <0.01);The expression levels of FGFR1 protein increased from 12 days old, reached the highest at 17 days old, and then decreased. The expression levels of FGFR 2 mRNA at 1, 5, 8, 12, 21 and 23 days old were significantly lower than at 17 days old( P <0.01), and the expression at 3 days old was lower than at 17 days old, the difference was not significant( P >0.05);FGFR2 protein was highly expressed at 1-17 days old. The results showed that during the first hair follicle growth cycle of mice, FGF21 plays an important role in the proliferation and differentiation of fibroblasts involved in hair follicle formation, and induces hair follicles to enter the catagen from the anagen. FGFR1 plays an important role in the proliferation and differentiation of inner and outer root sheath cells, and induces hair follicles to enter the telogen from the catagen. FGFR2 plays an important role in cell proliferation and differentiation in hair follicles, and induces hair follicles to enter the telogen from the anagen and catagen.
作者
吴晋强
曹校瑞
闫瑞琴
陆娜
张娇娇
吴佳豪
赫晓燕
WU Jinqiang;CAO Xiaorui;YAN Ruiqin;LU Na;ZHANG Jiaojiao;WU Jiahao;HE Xiaoyan(College of Animal Science and Veterinary Medicine,Shanxi AgriculturalUniversity,Taigu 030801,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2019年第3期534-543,共10页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
山西省自然科学基金(201701D121106)
