摘要
目的采用高效液相色谱法测定柴香疏肝胶囊中芍药苷的含量。方法采用高效液相色谱法,色谱柱为Inert Sus-tain C18柱(4. 6mm×150 mm,5μm),以乙腈-0. 1%磷酸溶液(14∶86)为流动相,流速为1. 0 m L/min,检测波长为230nm。结果芍药苷的线性范围为0. 1172～2. 344μg(r=0. 9999),芍药苷平均加样回收率为99. 05%,RSD为2. 08%。结论高效液相色谱法可以用于柴香疏肝胶囊中芍药苷的含量测定。
ObjectiveTo determinate the content of Paeoniflorin in Chaixiangshugan Capsule by HPLC.MethodsHigh performance liquid chromatography(HPLC)method was used,which applied InertSustain-C18 column(4.6 mm×150 mm,5μm)and acetonitrile-0.1%orthophosphoric(14∶86)as mobile phase.The flow rate was 1.0 mL/min,and detection wavelength was 230 nm.ResultsThe linear range was 0.1172~2.344μg and correlation coefficient was 0.9999.The average recovery was 99.05%,and the relative standard deviation was 2.08%.ConclusionHPLC method can be applied to the determination of Paeoniflorin in Chaixiangshugan Capsule.
作者
肖露
李淑翠
栾海云
张树平
XIAO Lu;LI Shucui;LUAN Haiyun;ZHANG Shuping(Department of Pharmacology,School of Pharmacy,BinZhou Medical University,Yantai 264003,P.R.China;Department of Pharmacy,Heze Hospital of Traditional Chinese Medicine)
出处
《滨州医学院学报》
2019年第1期30-32,共3页
Journal of Binzhou Medical University
关键词
柴香疏肝胶囊
芍药苷
液相色谱法
含量测定
Chaixiangshugan Capsule
Paeoniflorin
HPLC
content determination.
