摘要
为僵蚕人工饲养提供优良菌株,并提高僵蚕的产量和质量,采用形态学观察与分子生物学相结合的方法对从自然感病僵蚕上分离的致病菌株进行鉴定,并研究其人工饲养僵蚕的最适使用浓度。结果表明:该菌株为球孢白僵菌(Beauveria bassiana NB),其最适应用浓度为(1～5)×10~7个/mL,该浓度家蚕僵化死亡率及僵蚕平均单条重量最高,分别为98%和0.9g,僵蚕收益率最大化。
The pathogenesis strain isolated from stiff silkworm naturally infected Beauveria bassiana was identified by using morphological observation and molecular biology and the optimum concentration for artificial feeding of Bombyx batryticatus was studied to provide good strains for artificial feeding of B.batryticatus and improve yield and quality of B.batryticatus. Result: The isolated strain is identified as B.bassiana NB and the optimum application concentration for artificial feeding of B.batryticatus is (1~5)×10^7.The inactivation mortality rate and average weight/stiff silkworm of B.mori larvae inoculated with B.bassiana NB with the optimum application concentration are 98% and 0.9 g (the highest) respectively and the yield rate of B.batryticatus can reach the maximum.
作者
李文学
青学刚
陈宝瑞
肖文福
邹邦兴
刘俊凤
LI Wenxue;QING Xuegang;CHEN Baorui;XIAO Wenfu;ZHOU Bangxing;LIU Junfeng(Sericultural Research Institute,Sichuan Academy of Agricultural Sciences,Nanchong,Sichuan 637000,China)
出处
《贵州农业科学》
CAS
2019年第3期93-95,共3页
Guizhou Agricultural Sciences
基金
四川省农业科学院青年基金项目"药用白僵蚕人工培养技术研究"(2016QNJJ-016)
四川省农业科学院创新能力提升工程项目"蚕桑病虫害绿色防控关键技术研究"(2016GYSH-029)
四川省农业厅农业创新团队项目"四川蚕桑创新团队-家蚕疫病防控与饲育技术研发岗位"
关键词
家蚕
僵蚕
球孢白僵菌
人工饲养
Bombyx mori
Bombyx batryticatus
Beauveria bassiana
artificial feeding
