摘要
【目的】分离鉴定多穗柯炭疽病病原,并筛选出最佳防治药剂,为多穗柯炭疽病的识别和防治提供理论依据。【方法】采用组织块分离法分离多穗柯炭疽病病原菌,通过柯赫氏法则确定致病菌,结合形态学和分子生物学对多穗柯炭疽病病原菌进行鉴定;采用菌丝生长速率法测定8种不同杀菌剂对多穗柯炭疽病菌的室内毒力,根据供试杀菌剂的半数有效浓度(EC50)筛选出最佳的防治药剂。【结果】引起多穗柯炭疽病的病原为暹罗炭疽菌(Colletotrichum siamense);不同杀菌剂对多穗柯炭疽病菌菌丝生长的抑制效果存在一定差异,其中98.4%多菌灵的抑菌效果最强,所有供试浓度对多穗柯炭疽病菌的抑制率均为100.00%;其次是97%吡唑醚菌酯和98%福美双,EC50分别为0.04和0.05mg/mL。【结论】多穗柯为暹罗炭疽菌的新寄主。98.4%多菌灵、97%吡唑醚菌酯和98%福美双可作为防治多穗柯炭疽病的化学杀菌剂。
【Objective】The purpose of the study was to isolate and determine the pathogen of Lithocarpus polystachyus Rehd.anthracnose,and to screen appropriate fungicides against it,and provide reference for identification and control of L.polystachyus anthracnose.【Method】Tissue block separation method was used for the isolation of the pathogen of L.polystachyus anthracnose.Koch’s rule was used for the confirmation of the pathogen.The pathogen was further identified by morphological and molecular biology methods.The indoor toxicity tests of eight fungicides on pathogen of L.polystachyus anthracnose were determined by mycelium growth rate method.The optimal control fungicides were selected based on median effective concentration(EC50)of the fungicides.【Result】L.polystachyus anthracnose was caused by Colletotrichum siamense,and different fungicides displayed different inhibitory effects on mycelial growth of C.siamense.98.4% carbendazim showed the best inhibitory activities,and the inhibition rate were 100.00% against C.siamense in all the test concentrations.97% pyraclostrobin and 98% thiram also performed inhibitory activities,and the EC50 values were 0.04 and 0.05 mg/mL respectively.【Conclusion】L.polystachyus is a new host for C.siamense.98.4% carbendazim,97% pyraclostrobin and 98% thiram can be used as fungicides against C.siamense.
作者
宋慧云
段志豪
韦嘉怡
王军
毛子翎
单体江
SONG Hui-yun;DUAN Zhi-hao;WEI Jia-yi;WANG Jun;MAO Zi-ling;SHAN Ti-jiang(College of Forestry and Landscape Architecture,South China Agricultural University/Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm,Guangzhou 510642,China;South China Agricultural University/Guangdong Province Key Laboratory of Microbial Signals and Disease Control,Guangzhou,510642,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2019年第3期593-599,共7页
Journal of Southern Agriculture
基金
广东省林业科技创新项目(2015KJCX043)
关键词
多穗柯
炭疽病
病原
室内毒力测定
Lithocarpus polystachyus Rehd.
anthracnose
pathogen
indoor toxicity measurement
