摘要
为了深入了解苹果受潜隐性病毒的影响情况,本研究针对云南、贵州、四川三省的387份苹果叶片样本,利用RT-PCR及多重RT-PCR技术,分析鉴定了三种苹果潜隐性病毒——苹果茎痘病毒(ASPV)、苹果茎沟病毒(ASGV)和苹果褪绿叶斑病毒(ACLSV)在苹果上的发生现状.结果显示,苹果样本中ASPV阳性率为89.4%,ASGV阳性率为100%,ACLSV阳性率为81.6%,且它们多为混合感染,共计304份样本同时感染了三种病毒,混合感染率为78.5%.与云南、贵州相比,采自四川的苹果样本受潜隐性病毒感染最为严重.本研究报道了我国西南苹果主产区潜隐性病毒感染现状,建立了一套快速有效的苹果潜隐性病毒分子生物学鉴定方法.
In order to gain a further study of the impact of apples on the latent virus, 387 samples of apple leaves from the provinces of Yunnan, Sichuan and Guizhou were collected in this study. Three kinds of apple latent viruses, including ASPV(Apple stem pitting virus), ASGV (Apple stem grooving virus) and ACLSV (Apple chlorotic leaf spot virus), were analyzed and identified by RT-PCR (Reverse Transcription PCR) and multiplex RT-PCR. The results demonstrated that the incidence rate of ASPV, ASGV, and ACLSV was 89.4%, 100%, and 81.6%, respectively. In addition, 304 out of 387 samples were co-infected by all three viruses. Compared with Yunnan and Guizhou provinces, apple samples collected from Sichuan province are most seriously infected by the apple latent viruses. The status of latent viruses infection in the main apple producing areas in Southwest China is first reported in this study, a set of rapid and effective molecular method for latent viruses identification is established.
作者
姚润东
史文森
孙吴润泽
黄奎
王健美
YAO Run-Dong;SHI Wen-Sen;SUNWU Run-Ze;HUANG Kui;WANG Jian-Mei(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China)
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2019年第2期357-362,共6页
Journal of Sichuan University(Natural Science Edition)
基金
国家转基因重大专项(2016ZX08009003-002)
