摘要
目的研究干扰整合素转接激酶(ILK)表达对口腔鳞癌细胞迁移和侵袭能力的影响及机制。方法以口腔鳞癌SCC-25细胞为研究对象,细胞转染小干扰RNA阴性对照(siRNA control)和ILK小干扰RNA(ILK siRNA)分别记为si-NC组和ILK siRNA组,同时以没有转染的SCC-25细胞作为对照组。RT-PCR和Western blot法检测转染效果;噻唑蓝(MTT)检测细胞增殖;细胞划痕实验检测细胞迁移; Transwell小室检测细胞侵袭; Western blot法检测基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)表达。结果 si-NC组ILK mRNA水平和蛋白水平、细胞存活率、细胞迁移率、细胞侵袭数目以及细胞中的MMP-9、MMP-2蛋白表达与对照组比较差异无统计学意义(P> 0. 05)。ILK siRNA组ILK mRNA水平和蛋白水平、细胞存活率、细胞迁移率、细胞侵袭数目和细胞中MMP-2、MMP-9蛋白表达水平明显低于对照组和si-NC组(P <0. 05)。结论干扰ILK能够下调口腔鳞癌细胞增殖、迁移及侵袭能力。
Objective To investigate the effect and its mechanism of integrin-linked kinase(ILK)on the migration and invasion of oral squamous cell carcinoma cells.Methods The oral squamous cell carcinoma SCC-25 cells were studied,the cells transfected with siRNA control and ILK siRNA were recorded as si-NC group and ILK siRNA group,the SCC-25 cells without transfection were used as controls.Transfection effects were detected by RT-PCR and Western blot.Cell proliferation was detected by MTT.Cell scratch test was used to detect cell migration.Cell invasion was detected by Transwell chamber.The expression of MMP-2 and MMP-9 was detected by Western blot.Results The levels of ILK mRNA and protein level,cell survival rate,cell migration rate,the number of cell invasion and the expression of MMP-2,MMP-9 in si-NC group were not significantly different from those in the control group(P>0.05).The levels of ILK mRNA and protein level,cell survival rate,cell migration rate,the number of cell invasion and the expression of MMP-2,MMP-9 in ILK siRNA group were decreased significantly from those in the control group and si-NC group(P<0.05).Conclusion Interference with ILK inhibits the proliferation,migration and invasion of oral squamous cell carcinoma cells,and its mechanism may be related to the inhibition of MMP-2 and MMP-9 expression.
作者
宋玮华
刘月华
关超
李强
Song Weihua;Liu Yuehua;Guan Chao(Department of Stomatology,Yueyang Hospital of Integrated Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)
出处
《医学研究杂志》
2019年第3期77-81,共5页
Journal of Medical Research
基金
上海市科学技术委员会科研计划项目(16ZR1601021)
关键词
整合素转接激酶
口腔鳞癌
迁移
侵袭
Integrin-linked kinase
Oral squamous cell carcinoma
Migration
Invasion
