摘要
目的:制备具有碱性成纤维细胞生长因子缓释性能手术缝纫线的复合材料,并探究其机械性能和体外释药情况。方法:利用静电纺丝技术,通过研究复合材料中物质成分的配比,优化包载效率,并研究聚己内酯-胶原复合结构(PCL-Col)对可控释放bFGF的影响来制备具有生物活性与药物缓释性能的手术缝纫线;观察缝纫线微观结构,检测缝纫线机械性能和载药量。结果:通过扫描电镜结果证明载药的手术缝纫线具有完整的线状结构。进一步通过拉伸与拉伸循环实验证明缝纫线具有良好的机械性能,最后通过酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)证明所制备的手术缝纫线具备一定的药物缓释性能。结论:通过静电纺丝装置,优化纺丝参数,成功制备了PCL-Col-bFGF的手术缝线复合材料。该缝纫线满足缝合所需要的机械性能同时具备一定的bFGF缓释性能。
Objective:Composite materials that surgical sewing thread with basic fibroblast growth factor slow release performance were prepared,and exploring its mechanical properties and in vitro release.Methods:By means of electrospinning technology,the efficiency of packaging was optimized by studying the ratio of material composition in composite materials.Furthermore,the influence of polycaprolactone-collagen composite structure(PCL-Col)on the controllable release of bFGF was studied,to prepare the surgical sewing thread with biological activity and drug sustained release performance.Finally,the microstructure of the sewing thread was observed,and the mechanical properties and the drug loading capacity were measured.Results:The results of scanning electron microscopy(SEM)showed that the surgical sewing thread had a complete linear structure.It is proved that the sewing thread has good mechanical properties by stretching and stretching cycle experiments.Finally,enzyme linked immune sorbent assay(enzyme linked immunosorbent assay,ELISA)proved that the surgical sewing thread had a certain of drug release properties.Conclusion:By using electrospinning device and optimizing spinning parameters,PCL-Col-bFGF surgical suture composite was successfully prepared.The sewing thread meets the mechanical properties requirement of suturing and has b FGF sustained release performance.
作者
宋奕
张翠云
李奕
张素素
潘舜
陶云云
许璐摇
何华成
吴疆
Yi SONG;Cui-yun ZHANG;Yi LI;Su-su ZHANG;Shun PAN;Yun-yun TAO;Lu-yao XU;Hua-cheng HE;Jiang WU(School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou 325035,China;College of Chemistry and Materials Engineering,Wenzhou University,Wenzhou 325035,China)
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2019年第1期55-62,共8页
China Biotechnology
基金
2018年浙江省大学生科技创新活动计划暨新苗人才计划大学生科技创新项目(2018R413019)
国家级大学生创新创业训练计划项目(201810343001)资助项目
关键词
静电纺丝
生长因子
机械性能
缓释性能
Electrospinning
Growth factor
Mechanical property
Slow release performance
