摘要
目的探讨有氧运动改善脑小血管病大鼠认知执行能力的效果及作用机制。方法 8周龄雄性Sprague-Dawley大鼠随机分为假手术组(n=16)、模型组(n=16)和游泳组(n=16)。双侧颈动脉结扎法造模。4周游泳锻炼后,各组行挖穴测试。Western blotting检测海马脑源性神经营养因子(BDNF)和原肌球蛋白受体激酶B (TrkB)蛋白水平。高尔基染色法观察海马神经元树突及突触棘的发育情况。免疫荧光法检测海马齿状回Ki67、双皮质素(DCX)和Neun的表达。结果与模型组相比,游泳组挖穴能力更强(P <0.05),海马组织中BDNF和TrkB蛋白水平更高(P <0.05),海马齿状回Ki67/DCX和Neun阳性细胞表达率更高(P <0.05),海马区神经元树突范围更大,突触棘更长,神经元发育更好(P <0.05)。结论有氧运动通过BDNF/TrkB信号通路促进海马神经元的增殖分化,增加Ki67/DCX和Neun表达,促进海马神经元发育,改善脑小血管病大鼠执行功能。
Objective To explore the effect of aerobic exercise on behavioral function in rats with cerebral small vessel disease and its mechanism. Methods Eight-week-old male Sprague-Dawley rats were randomly divided into sham group (n = 16), model group (n = 16) and swimming group (n = 16). The model was developed with bilateral common carotid artery ligation. They were assessed with burrowing test after four weeks of swimming exercise. The levels of brain-derived neuro-trophic factor (BDNF) and tropomyosin receptor kinase B (TrkB) protein in hippocampus were detected with Western blotting. The development of dendrites and synaptic spines in hippocampal neurons was observed with Golgi staining. The expression of Ki67, doublecortin (DCX) and Neun in hippocampal dentate gyrus was detected with immunofluorescence. Results Compared with the model group, the burrowing ability improved in the swimming group (P < 0.05), with increase of levels of BDNF and TrkB in hippocampus (P < 0.05), Ki67/DCX and Neun positive cells in hippocam-pal dentate gyrus (P < 0.05), and extension of dendrites and length of synaptic spine in hippocampal neurons (P < 0.05). Conclusion Aerobic exercise may promote the proliferation and differentiation of hippocampal neurons through BDNF/ TrkB signaling pathway, expression of Ki67 / DCX and Neun and development of hippocampal neurons, to improve behavioral function in rats with cerebral small vessel disease.
作者
王茹
刘楠
李卫萍
赵弘轶
杜菊梅
黄勇华
WANG Ru;LIU Nan;LI Wei-ping;ZHAO Hong-yi;DU Ju-mei;HUANG Yong-hua(Department of Neurology, the Seventh Medical Center of PLA General Hospital, Beijing 100700, China;Departmentof Neurology, Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang, Shaanxi 712000,China)
出处
《中国康复理论与实践》
CSCD
北大核心
2019年第3期249-254,共6页
Chinese Journal of Rehabilitation Theory and Practice
基金
国家自然科学基金青年项目(No.81601035)
National Natural Science Foundation of China(Youth)(No.81601035)
关键词
脑小血管病
有氧运动
海马
执行功能
脑源性神经营养因子
原肌球蛋白受体激酶B
大鼠
cerebral small vessel disease
aerobic exercise
hippocampus
behavioral function
brain-derived neurotrophic factor
tropomyosin receptor kinase B
rats
