摘要
以3%葡聚糖硫酸钠(DSS)为诱导剂建立ICR小鼠结肠炎症模型,评估米糠多糖的抗炎功效与分子机理。采用病理组织分析、RT-qPCR和Western blotting等多种技术比较各组动物组织的病理改变和基因表达的差异。结果发现:给予500mg/(kg·d)米糠多糖灌胃能明显改善结肠炎症小鼠的整体健康状况,降低疾病活动指数分数,减轻小鼠结肠炎症组织的损伤,改善炎症相关生化指标。RT-qPCR分析发现,米糠多糖可使结肠组织炎症因子TNFα、IL-1β、Cox-2和iNOS等基因mRNA表达水平下调,Western blotting分析进一步证实了米糠多糖抑制炎症因子蛋白表达水平的功效,同时,米糠多糖也能抑制MAPK信号通路。结果表明米糠多糖具有抗炎作用,其分子机制可能与下调炎症因子的表达和阻断MAPK信号通路相关。
To assess the anti-inflammatory function and possible molecular mechanism of rice bran polysaccharide(RBP),DSS-induced colitis model was used in ICR mice.Pathological changes and gene expression were analyzed by histopathological analysis,RT-qPCR and Western blotting among of different experiment groups.The results showed that 500 mg/kg/day RBP could significantly improve the health status of mice and reduced colonic inflammation.RBP reduced the disease active index(DAI)score of DSS-induced mice and alleviated the damage of pathological tissue,with the improvement of the inflammation-related biochemical indicators.qRT-PCR analysis showed that RBP could decrease the mRNA expression level of inflammatory factors,including TNFα,IL-1β,Cox-2 and iNOS in colon tissues.Western blotting analysis further confirmed the efficacy of RBP inhibiting the expression level of inflammatory factor protein.Moreover,RBP could also inhibit MAPK signaling pathway.The results suggested that RBP had anti-inflammatory effect,and its molecular mechanism might be related to down-regulating the expression of inflammatory factors via inhibiting MAPK signaling pathway.
作者
刘晶
郭婷
郭天一
邹佳文
周潇恬
何洁
石雨虹
罗非君
LIU Jing;GUO Ting;GUO Tian-yi;ZOU Jia-wen;ZHOU Xiao-tian;HE Jie;SHI Yu-hong;LUO Fei-jun(College of Chemical Biology and Environmental Engineering,Xiangnan University,Chenzhou,Hunan423000,China;Hunan Key Laboratory of Grain and Oil Deep Processing and Quality Control,College ofFood Science and Engineering,Central South Forestry University,Changsha,Hunan 410004,China;National Engineering Laboratory of Rice and By-Products Deep Processing,College of FoodScience and Engineering,Central South Forestry University,Changsha,Hunan 410004,China)
出处
《食品与机械》
北大核心
2019年第1期32-40,共9页
Food and Machinery
基金
湖南省教育厅科研项目(编号:湘教395 16C1496)
湖南省"2011"协同创新课题(编号:湘教通[2013]448号)
关键词
米糠
多糖
炎症
炎症因子
MAPK
rice bran
polysaccharide
inflammation
inflammatory factors
MAPK
