摘要
采用硅胶、反向硅胶、Sephadex LH-20及MCI柱色谱等多种色谱技术和方法对滇南乌头Aconitum austroyunnanense进行化学成分研究,并利用1H-NMR,13C-NMR和MS等波谱学方法并结合文献对分离得到的化合物进行结构鉴定。从该植物地下根的95%乙醇提取液的乙酸乙酯萃取部分中分离得到8个C_(19)二萜生物碱,分别鉴定为14α-benzoyloxy-13β,15α-dihydroxy-1α,6α,8β,16β,18-pentamethoxy-19-oxoaconitan (1),N-deethylaconitine (2),spicatine B (3),leucanthumsine A (4),acofamine B(5),macrorhynine B(6),aconitilearine(7)和ambiguine(8)。其中,化合物1为新化合物,化合物2～8为首次从该植物中分离得到。采用MTT比色法对部分生物碱进行体外抗肿瘤活性测试,实验结果表明化合物1对人类肿瘤细胞系A-549,Hep G2,He La具有一定的细胞毒活性(IC_(50)<20μmol·L^(-1))。
Eight Cf9-diterpenoid alkaloids ( 1-8) were isolated from the ethyl acetate soluble fraction of 95% ethanol extract of ihr ground roots of Aconitum austroyunnanense through various column chromatographies on silica gel, ODS, Sephadex LH-20 and MCI gel. Their structures were elucidated as 14a-henzoyloxy-13β, 15a-dihydroxy-1 a, 6a, 8β, 16^8, 18-pentamethoxy-19-oxoaconitan ( 1), TV-deethylaconitine ( 2), spicatine B (3), leucantliumsine A (4), acofamine B (5), macrorhynine B (6), aconitilearine (7), and ambiguine (8) based on their chemical and physicochemical properties and spectroscopic data. Compound 1 was a new compound and alkaloids 2-8 were isolated from this plant for the first time. Some isolated alkaloids were tested in vitro for cytotoxic potential by employing the MTT method. As a result, alkaloid 1 exhibited weak cytotoxic activity against three tested tumor cell lines ( A-549, HeLa, and HepG2) with IC50values less than 20 μmol· L^-1.
作者
胡疆
吕涛
蔡建
高秀
张丽芳
景年华
彭天凤
史俊友
郝珊瑚
HU Jiang;LYU Tao;CAI Jian;GAO Xiu;ZHANG Li-fang;JING Nian-hua;PENG Tian-feng;SHI Jun-you;HAO Shan-hu(Institute of Characteristic Medicinal Resource of Ethnic Minorities , Qujing Normal University, Qujing 65501 1 , China;IMuclear Medicine Department, General Hospital of Shenyang Military Region , Shenyang 110016, China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第4期717-722,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(31760341
31801175)
云南省应用基础研究计划项目(2016FD080)
关键词
滇南乌头
毛茛科
化学成分
二萜生物碱
抗肿瘤活性
Aconitum austroyunnanense
Ranunculaceae
chemical constituted
diterpenoid alkaloids
cytotoxicity
