摘要
目的探讨miRNA-323对胃癌细胞SGC-7901的增殖作用。方法使用qRT-PCR检测miRNA-323在胃癌组织中的表达情况,进一步转染miRNA-323 inhibitor和miRNA-323 mimic到胃癌细胞SGC-7901中,分别使用qRT-PCR、Western blot和流式细胞仪探究miRNA-323对SGC-7901的增殖作用,以及细胞周期蛋白Cyclin D1和癌基因C-myc的表达量。结果 qRT-PCR结果显示,miRNA-323在胃癌组织中显著高于癌旁组织(P<0.05)。在胃癌细胞SGC-7901中抑制miRNA-323表达后,Cyclin D1和C-myc蛋白表达明显低于正常组(P<0.05),且S期细胞显著降低;增加miRNA-323表达后,Cyclin D1和C-myc蛋白表达明显高于正常组(P<0.05),且S期细胞显著升高。结论抑制miRNA-323表达能够抑制胃癌细胞SGC-7901的增殖。
Objective To investigate the effect of miRNA-323 on the proliferation of gastric cancer cell line SGC-7901.Methods qRT-PCR was used to detect the expression of miRNA-323 in gastric cancer tissues,and miRNA-323 inhibitor and miRNA-323 mimics were further transfected into gastric cancer SGC-7901 cells.Effect of miRNA-323 on proliferation of SGC-7901 and the expression of Cyclin D1 and oncogene C-myc was observed by qRT-PCR,Western blot and flow cytometry.Results The results of qRT-PCR showed that miRNA-323 was significantly higher in gastric cancer tissues than in adjacent tissues(P<0.05).After inhibiting the expression of miRNA-323 in gastric cancer SGC-7901 cells,the expression of Cyclin D1 and C-myc protein was significantly higher than that in the normal group(P<0.05),and the cells in S phase were significantly increased.After increasing the expression of miRNA-323,the expression of Cyclin D1 and C-myc protein was significantly higher than that of the normal group(P<0.05),and the cells in S phase were significantly increased.Conclusion miRNA-323 can inhibit the proliferation of gastric cancer cell line SGC-7901.
作者
陈鹤
卢杨
赵燕
余焱霞
王中新
张敏
Chen He;Lu Yang;Zhao Yan(Dept of Clinical Laboratory,The First Affiliated Hospital of Anhui Medical University,Hefei 230022;Dept of Clinical Laboratory,The Affiliated Maternal and Child Health Hospital of Anhui MedicalUniversity/Anhui Province Maternal and Child Health Hospital,Hefei 230001)
出处
《安徽医科大学学报》
CAS
北大核心
2019年第4期520-524,共5页
Acta Universitatis Medicinalis Anhui
基金
安徽省高等学校自然科学研究重点项目(编号:KJ2017A208)
