摘要
目的探讨Raf激酶抑制蛋白(RKIP)、P27蛋白在鼻咽癌组织中的表达及与鼻咽癌发生、发展的关系。方法选取2012年3月—2016年1月南华大学附属第一医院病理科收集的鼻咽癌黏膜组织76例(病例组)和正常鼻咽组织30例(对照组),采用免疫组织化学检测两组标本中的RKIP、P27蛋白表达水平,并分析RKIP、P27蛋白与鼻咽癌发生、发展的关系。结果两组RKIP蛋白和P27蛋白阳性表达率比较,差异有统计学意义(P <0.05),病例组的RKIP蛋白阳性表达率55.26%低于对照组的86.67%,病例组的P27蛋白阳性表达率77.63%高于对照组的46.67%;发生远处转移的鼻咽癌患者、Ⅲ期和Ⅳ期鼻咽癌患者、发生淋巴结转移的鼻咽癌患者病灶组织中的RKIP蛋白阳性表达率低于未发生远处转移的鼻咽癌患者、Ⅰ期和Ⅱ期鼻咽癌患者、未发生淋巴结转移的鼻咽癌患者,差异有统计学意义(P<0.05);发生远处转移的鼻咽癌患者、Ⅲ期和Ⅳ期鼻咽癌患者、发生淋巴结转移的鼻咽癌患者病灶组织中的P27蛋白阳性表达率高于未发生远处转移的鼻咽癌患者、Ⅰ期和Ⅱ期鼻咽癌患者、未发生淋巴结转移的鼻咽癌患者,差异有统计学意义(P <0.05)。结论鼻咽癌组织中RKIP蛋白低表达、P27蛋白高表达,并且与鼻咽癌肿瘤的发生、发展密切相关。
Objective To investigate the expression of Raf kinase inhibitor protein(RKIP)and P27 protein in nasopharyngeal carcinoma(NPC)and its clinical significance.Methods A total of 76 cases of nasopharyngeal carcinoma mucosa(case group)and 30 cases of normal nasopharyngeal tissue(control group)which were collected in our hospital from March 2012 to January 2016 were involved in this study.Immunohistochemistry was performed for detection of RKIP and P27 protein.Clinical significance of RKIP,P27 protein in nasopharyngeal carcinoma including occurrence and development were determined.Results The positive expression rates of RKIP and P27 in the two groups were statistically significant(P<0.05).The positive expression rate of RKIP in the case group(55.26%)was lower than that in the control group(86.67%).The positive expression rate of P27 in the case group(77.63%)was higher than the control group(46.67%);the positive expression rates of RKIP and P27 in lesions of NPC patients with distant or lymph node metastasis,stage III or IV nasopharyngeal carcinoma were significantly lower and higher than those with distant or lymph node metastasis,stage I or II,respectively;the differences were statistically significant(P<0.05).Conclusions Decrease of RKIP and increase of P27 may be associated with the occurrence and development of nasopharyngeal carcinoma.
作者
龚永谦
罗兴谷
唐洪波
Yong-qian Gong;Xing-gu Luo;Hong-bo Tang(Department of Otorhinolaryngology,The First Affiliated Hospital of University of South China,Hengyang,Hunan 421001,China)
出处
《中国现代医学杂志》
CAS
2019年第7期34-38,共5页
China Journal of Modern Medicine
基金
湖南省财政厅资助项目(湘财教指[2017]99号)
湖南省教育厅科研项目(No:16C1399)
