摘要
目的:制备琼脂糖/ε-聚赖氨酸(ε-PLL)/碳酸羟基磷灰石(CHA)新型牙周引导组织再生(GTR)屏障膜,并评价其生物相容性和抗菌性能。方法:首先采用水热法制备CHA,通过扫描电镜(SEM)和傅里叶红外光谱(FTIR)分析CHA的形态和构象;采用琼脂糖水凝胶包载CHA和ε-PLL制备屏障膜;流变仪检测屏障膜的机械性能;活细胞/死细胞(Live/Dead)染色检测生物相容性;抑菌环实验证实对金黄色葡萄球菌的抗菌性能。结果:SEM及FTIR分析表明成功合成中空柱状的CHA。CHA浓度的增加,显著增强其机械性能;Live/Dead染色结果显示屏障膜具有良好的生物相容性;在屏障膜周围可见明显的抑菌环。结论:成功制备琼脂糖/ε-PLL/CHA屏障膜,并证明其具有良好的生物相容性及抗菌性能。
Objective:To prepare a novel periodontal guided tissue regeneration(GTR)barrier membranes of agarose/ε-PLL/CHA and evaluation of it with excellent biocompatibility and antibacterial properties.Methods:CHA was prepared by the hydrothermal method.The characterization of the CHA were analyzed by Scanning electron microscopy(SEM)and Fourier transform infrared spectroscopy(FTIR).The different concentrations of CHA andε-PLL were added to the agarose to prepare the barrier membranes.The mechanical strength of barrier membranes was assessed through rheometer.Live/Dead staining was used to detect the biocompatibility of barrier membranes.The effect of antibacterial of barrier membranes was confirmed by inhibition zone.Results:SEM and FTIR indicated successful synthesis of hollow columnar CHA.Barrier membranes have good biocompatibility.CHA could enhance the mechanical properties of the barrier membranes.Live/Dead staining showed a good biocompatibility.There were obvious inhibition zones found around the barrier membranes.Conclusion:The agarose/ε-PLL/CHA barrier membrane was successfully prepared,and the membrane was proved to have good biocompatibility and antibacterial properties.
作者
何智琪
钱秋萍
邓辉
胡荣党
HE Zhiqi;QIAN Qiuping;DENG Hui;HU Rongdang(Department of Orthodontics,Hospital of Stomatology,Wenzhou Medical University,Wenzhou 325027,China;Department of Biomaterials,Wenzhou Institute of Biomaterials & Engineering,Wenzhou 325000,China;Department of Periodontics,Hospital of Stomatology,Wenzhou Medical University,Wenzhou 325027,China)
出处
《温州医科大学学报》
CAS
2019年第4期267-271,共5页
Journal of Wenzhou Medical University
基金
浙江省公益技术应用研究项目(LGF18H140007
LGF19H140001)
关键词
屏障膜
琼脂糖
聚赖氨酸
碳酸羟基磷灰石
抗菌
barrier membrane
agarose
poly-L-lysine
carbonated hydroxyapatite
antibacterial
