摘要
目的:采用行为学以及胃肠动力指标对大鼠经脉不通证候模型进行评价,为探索中医经络病候模型提供评价方法和基础。方法:采用随机数字表法将41只大鼠随机分为空白对照组10只、非经结扎组10只,胃经结扎组10只和肾经结扎组11只。空白对照组大鼠只进行麻醉处理,其余3组大鼠均用结扎法在相应的非经脉线或经脉线上进行结扎堵塞低流阻通道造模。采用旷场实验、糖水偏好实验观察行为学变化,通过计算胃残留率及小肠推进率观察胃肠动力变化。结果:胃经结扎组大鼠在造模后第1周行为学得分显著低于空白对照组(P<0.05),在造模后第4、5周显著高于空白对照组(P<0.05);肾经结扎组大鼠在造模后第1、2、4、5周行为学得分显著低于空白对照组(P<0.01或P<0.05);胃经结扎组大鼠在造模后第1周行为学得分显著低于非经结扎组(P<0.05),第5周显著高于非经结扎组(P<0.05);肾经结扎组大鼠在造模后每周行为学得分均显著低于非经结扎组(P<0.05或P<0.01)。胃经结扎组和肾经结扎组大鼠在造模后第1周中央区停留时间均显著少于空白对照组和非经结扎组(P<0.05)。胃经结扎组大鼠在造模后糖水偏好值明显高于空白对照组和非经结扎组(P<0.01)。胃经结扎组大鼠小肠推进率明显高于空白对照组和非经结扎组(P<0.05)。结论:结扎堵塞大鼠足阳明胃经与足少阴肾经循经低流阻通道,可导致与胃经、肾经病候相关的情志状态与胃肠动力改变,说明结扎法能引起大鼠相对特异性变化。本实验为经络疾病证候模型提供了评价手段和基础。
Objective: Behavioral and gastrointestinal motility indexes were used to evaluate the syndrome model of meridian obstruction in rats, which provided evaluation methods and basic information for exploring the syndrome model of meridian diseases in TCM. Method: 41 male SD rats were randomly divided into control grou p (CG, n=10), Ligation on non-meridian group (LNMG, n=10), Ligation on The Stomach Meridian group (LSTMG, n=10) and Ligation on The Kidney Meridian group (LKIMG, n=11). The CG was only given anesthesia. The oth-er three groups were tied on the non-meridian area or meridian area to blocking the low hydraulic resistance channels (LHRC). Weekly open field and sugar water preference experiment which was observed before and after modeling were conducted to observe behavioral changes in each group. Gastrointestinal motility index including gastric residual rate and small intestinal propulsion rate were observed after modeling. Results: In behavior score aspect, compared with the CG, the LSTMG was decreased significantly in the first week (P<0.05), and increased significantly in the fourth and fifth weeks (P<0.05);the LKIMG was decreased significantly in the first, second,fourth and fifth weeks (P<0.01 or P<0.05);compared with the LNMG, the LSTMG showed a significant decrease in the first week (P<0.05), and a significant increase at the fifth week (P<0.05);the LKIMG was decreased sig-nificantly every week after blocking meridians (P<0.05 or P<0.01). In the residence time of the central area as-pect, a significantly decreased were showed in both the LSTMG and LKIMG in the first week after the estab-lishment of the model (P<0.05). Compared with the CG and the LNMG, the sugar preference value of the LST-MG was significantly increased (P<0.01), and the small intestinal propulsion rate of the LSTMG was significantly higher than that of the CG and the LNMG (P<0.05). Conclusion: Blocking LHRC along the stomach meridian and kidney meridian by ligation resulted in changes of emotional statement and gastrointestinal motility, indicat-ing that the ligation method could induce relatively specific effects in rats. This experiment provides an evalua-tion method and foundation for syndrome models of meridians.
作者
叶丰瑶
张维波
王燕平
贾术永
宋晓晶
李宏彦
顾鑫
熊枫
YE Feng-yao;ZHANG Wei-bo;WANG Yan-pin;JIA Shu-yong;SONG Xiao-jing;LI Hong-yan;GU Xin;XIONG Feng(Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China;School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China)
出处
《中医药导报》
2019年第6期26-30,共5页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(81173206)
2018年度北京中医药大学在读研究生项目(2018-JYBZZ-XS109)
关键词
经脉不通
足阳明胃经
足少阴肾经
经络病候
行为学
胃肠活动
中医证候模型
大鼠
meridian obstruction
The Stomach Meridian
The Kidney Meridian
meridian disease
behavior
gastrointestinal motility
TCM model
rats
