摘要
目的检测胃癌患者肿瘤组织及血浆miR-216a的表达水平,探讨其与临床病理特征和手术治疗预后的关系。方法选取2012年1月至2014年12月在该院接受手术治疗的胃癌患者120例,收集肿瘤和癌旁组织标本,采集手术前后患者血浆和健康志愿者(120例)血浆。采用实时荧光定量PCR法检测miR-216a在组织和血浆标本中的表达情况,统计分析miR-216a表达水平与胃癌患者临床病理特征和手术预后的关系。结果 miR-216a在胃癌组织中的表达水平低于癌旁组织(P<0.01)。手术前,胃癌患者血浆miR-216a表达水平高于健康人群(P<0.01);术后,患者血浆miR-216a表达水平较手术前明显降低(P<0.05)。胃癌组织中miR-216a相对表达水平与肿瘤分化程度和TNM分期相关(P<0.05)。患者血浆miR-216a相对表达水平与肿瘤分化程度、TNM分期、淋巴结转移和肿瘤大小均相关(P<0.05)。胃癌组织miR-216a高表达患者术后复发率和生存率优于胃癌组织miR-216a低表达患者,差异无统计学意义(P>0.05);血浆miR-216a低表达患者的复发率和生存率优于血浆miR-216a高表达患者,差异有统计学意义(P<0.05)。结论胃癌患者肿瘤组织和血浆中miR-216a的表达变化差异明显,与肿瘤分化和分期等相关,且在血浆中的表达与手术预后相关,在胃癌早期诊治和预后判断中有潜在价值。
Objective To detect tumor tissue and plasma miR-216a expression levels in patients with gastric cancer,and explore its relationship with clinicopathological characteristics and prognosis after surgery.Methods A total of 120 cases of patients with gastric cancer undergoing surgical treatment in this hospital from January 2012 to December 2014 were selected.Tumor tissue and adjacent tissue samples,plasma specimens of patients before and after surgery,and plasma specimens of healthy volunteers(120 cases)were collected.The expression levels of miR-216a in tissue and plasma samples were detected by RT-qPCR,and their relationships to clinicopathological characteristics and prognosis of gastric cancer were statistically analyzed.Results The expression level of miR-216a in gastric cancer tissue samples was significantly lower than that in adjacent tissue samples(P<0.01).Before surgery,the plasma level of miR-216a in patients with gastric cancer was significantly higher than that in healthy volunteers(P<0.01).Compared with before surgery,the plasma level of miR-216a in patients with gastric cancer was significantly decreased after surgery(P<0.05).The relative expression level of miR-216a in gastric cancer tissues was correlated to tumor differentiation and TNM stage(P<0.05).The relative expression level of miR-216a in plasma was correlated to tumor differentiation,TNM stage,lymph node metastasis and tumor size(P<0.05).Patients with high expression of miR-216a in gastric cancer tissues had lower recurrence rate and higher survival rate than patients with low expression of miR-216a in gastric cancer tissues,no statistically significant difference was observed(P>0.05).However,patients with low plasma level of miR-216a had lower recurrence rate and higher survival rate than patients with high plasma level of miR-216a,there was statistically significant difference(P<0.05).Conclusion The expression of miR-216a in tissues and plasma of patients with gastric cancer is significantly different,which is related to tumor differentiation and staging,and its expression in plasma is related to the prognosis.The plasma miR-216a has potential value in early diagnosis and treatment and prognosis judgement of gastric cancer.
作者
张同
李庆
舒若
徐玉
施承民
曾玉剑
孙亮
田衍
肖博凯
王昆华
罗华友
ZHANG Tong;LI Qing;SHU Ruo;XU Yu;SHI Chengmin;ZENG Yujian;SUN Liang;TIAN Yan;XIAO Bokai;WANG Kunhua;LUO Huayou(Department of Gastrointestinal and Hernia Surgery,the First Affiliated Hospital of Kunming Medical University/Yunnan Institute of Digestive Diseases/Yunnan Engineering Technology Center of Digestive Disease,Kunming,Yunnan 650032,China)
出处
《重庆医学》
CAS
2019年第7期1155-1158,共4页
Chongqing medicine
基金
云南省消化病研究所内设机构项目(2016NS0085)
云岭学者项目(YLXL20170002)
云南省科技厅-昆明医科大学联合专项面上项目[2017FE467(-038)]
