摘要
目的比较G6PD表达敲减后人宫颈癌细胞HPV-C33A与HPV16+Siha以及HPV18+Hela中mi RNAs的表达差异,进一步了解G6PD致瘤的分子机制。方法构建G6PD表达敲减的人宫颈癌细胞株,运用mi RNAs芯片对各细胞株中mi RNAs进行对比分析,q RT-PCR对结果进行验证。结果 (1)成功获得G6PD表达敲减的人宫颈癌细胞株C33A、Siha和Hela;(2)与HPV-C33A相比,G6PD表达干扰后高危型HPV16+/18+人宫颈癌细胞株中出现多种mi RNAs差异表达的情况(P<0.05)。结论 G6PD在高危型HPV+人宫颈癌中的致瘤性与多种mi RNAs的差异表达相关。
Objective To explore the expression changes of miRNAs in HRHPV(16 and 18) infected(+) cervical cancer cells after G6 PD expression knock-down. Methods The G6 PD expression in HPV-C33 A,HPV16+Siha and HPV18+Hela cells was inhibited by RNA interference target G6 PD. Human micro RNA Microarray Kit and q RT-PCR were employed to determine the expression changes of mi RNA between different cell lines.Results The expression of G6 PD significantly decreased after RNA interference in different cervical cancer cell lines.Mi RNA microarrayand q RT-PCR analysis revealed that numerousmiRNAs in HPV+ Hela/Siha cells were expressional different from the control HPV-C33 A cells,following G6 PD knockdown. Conclusion The expression changes of various miRNAs following G6 PDknockdown are associated with the oncogenesis events of G6 PD in HR HPV infected cervical cancer cells.
作者
胡滔
刘光彩
洪颖
陈红兰
常业飞
姜水
檀雅欣
习杨彦彬
陈波
HU Tao;LIU Guang-cai;HONG Ying;CHEN Hong-lan;CHANG Ye-fei;JIANG Shui;TAN Ya-xin;XIYANG Yan-bin;CHEN Bo(Department of Laboratory Medicine,The Third People's Hospital of Yunnan Province,Kunming Yunnan 650011;Basic Medical College,Kunming Medical University,Kunming Yunnan 650500,China;Experiment Center for Medical Science Research,Kunming Medical University,Kunming Yunnan 650500,China)
出处
《昆明医科大学学报》
CAS
2019年第3期6-10,共5页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(81502377)
云南省应用基础研究计划资助项目(2016FB139)
云南省教育厅科学研究基金资助项目(2016ZDX044)
