摘要
生殖性能是评价水生动物亲本质量的重要指标,本研究测定和比较了野生和养殖中华绒螯蟹亲本的生殖性能、胚胎质量、胚胎色泽、常规生化和脂肪酸组成。结果显示:①野生中华绒螯蟹亲本的生殖力、生殖指数和抱卵量略高于养殖亲本的,但2组间无显著性差异,野生和养殖中华绒螯蟹亲本的胚胎单卵湿重、单卵干重和卵径无显著性差异;②养殖组冻干胚胎的红度(a~*)、黄度(b~*)值和总类胡萝卜素含量显著高于野生组亲本,然而2组胚胎的亮度(L~*)和色差值(dE~*)无显著性差异;③2组亲本所产胚胎的水分、粗蛋白和粗脂肪含量均无显著性差异,但其脂肪酸组成差异显著,野生组胚胎的C18:1n9、C18:1n7、C20:4n6、C22:5n3和C22:6n3显著高于养殖组,但其C18:2n6和C18:3n3含量显著低于养殖组。研究表明,池塘养殖和野生亲本的生殖性能和胚胎中常规生化组成无显著差异,但2组胚胎的色泽、总类胡萝卜素含量和部分脂肪酸组成存在显著差异,该研究为中华绒螯蟹亲本选择、生殖性能评价和人工育苗提供了重要基础资料。
The Chinese mitten crab(Eriocheir sinensis)is an important aquaculture species and food source in Eastern Asia.At the present,pond-reared broodstock are used for seed production of E.sinensis,and the reasonable utilization of wild-caught broodstock is conducive breeding and selection of E.sinensis.Reproductive performance is one of the most important indicators for the evaluation of broodstock quality in aquatic animals.This study was designed to determine and compare the reproductive performance,egg quality,colouration,proximate and fatty acid composition of wild-caught and pond-reared E.sinensis broodstock.The results showed that:①the wild-caught E.sinensis broodstock had the slightly higher egg production,fecundity and reproductive effort than the pond-reared females,but there was no significant difference between two treatments.The egg diameter,wet weight and dry weight of individual egg were similar between two treatments.②The eggs from pond-reared E.sinensis broodstock had significantly higher redness(a^*),yellowness(b^*)and total carotenoids contents than those of the eggs produced by the wild-caught broodstock,however,there are no significant differences for lightness(L^*)and colour difference(dE^*).③As for proximate composition of eggs,no significant differences were found for the moisture,total protein and total lipid contents between the wild-caught and pond-reared treatments.The eggs from wild-caught broodstock had the significantly higher percentages of C18:1n9,C18:1n7,C20:4n6,C22:5n3 and C22:6n3 than the eggs produced by the pond-reared broodstock,but the C18:2n6 and C18:3n3 of the wild caught broodstock were significantly lower than those of the pond-reared broodstock.In conclusion,these results indicated that the reproductive performance and egg proximate composition were similar between wild-caught broodstock and pond-reared broodstock with similar body weight,but significant differences were found on redness(a^*),yellowness(b^*),total carotenoids content and fatty acid composition in the eggs.These results could provide valuable information for the broodstock selection,evaluation of reproductive performance and artificial breeding of E.sinensis in the future.
作者
李清清
吴旭干
姜晓东
苏雨
郑海地
成永旭
LI Qingqing;WU Xugan;JIANG Xiaodong;SU Yu;ZHENG Haidi;CHENG Yongxu(Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Centre for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China;Zhejiang Aoling Aquacultrue Seed Industry Co.,Ltd.,Changxing 313106,China)
出处
《水产学报》
CAS
CSCD
北大核心
2019年第4期858-866,共9页
Journal of Fisheries of China
基金
现代农业产业技术体系专项(CARS-48)
上海市科技兴农推广项目[沪农科推字(2015)第1-7号]
上海市科委工程技术中心能力提升项目(16DZ2281200)
上海高校水产学高峰学科建设项目(2015-62-0908)
深圳市澳华农牧有限公司横向项目(D-8006-15-0054)~~
关键词
中华绒螯蟹
亲本来源
生殖性能
生化组成
类胡萝卜素
Eriocheir sinensis
broodstock source
reproductive performance
biochemical composition
carotenoids
