摘要
龙须菜已在我国沿海从南到北广泛栽培,但其栽培周期受夏季高温的限制。本研究采用qRT-PCR和Western blot技术研究了龙须菜核酮糖-1, 5-二磷酸羧化酶/加氧酶大亚基(rbcL)和热休克蛋白70(HSP70)对高温胁迫及3种抗逆植物激素的响应。结果显示:①高温(33°C)显著抑制了龙须菜rbc L转录和蛋白的表达水平,而100μmol/L水杨酸(SA100)和50μmol/L茉莉酸甲酯(MJ50)的添加可减轻高温的不利影响。在SA100和MJ50组中,rbc L转录表达量分别为高温组的1.31倍和1.32倍(3 h),rbcL蛋白表达量分别为高温组的1.36倍和2.10倍(24 h);并且这2种激素也能一定程度上缓解高温对藻体Rubisco活性的抑制作用,恢复Rubisco活化状态。但是50μmol/L脱落酸(ABA50)的添加则基本上抑制了rbcL表达及Rubisco活性。②3种激素进一步促进了高温诱导的龙须菜HSP70的表达,在激素添加后,其转录水平升高0.53～1.00倍(3 h),蛋白水平升高0.93～2.45倍(24 h)。可见,植物激素SA、MJ和ABA在调控由高温引起的光合作用酶的抑制和热休克蛋白的诱导表达方面发挥了一定的作用。
Seaweed Gracilariopsis lemaneiformis is mainly used as the bait for abalone and raw material for agar production in China.This macroalga is widely cultivated along the coasts of China from south to north,but its cultivation period is limited by high temperature in summer.In the current study,the effects of heat and phytohormone treatments on Rubisco large subunit(rbcL)and heat shock protein 70(HSP70)were investigated by using quantitative real-time PCR and Western blot technologies in G.lemaneiformis.The results showed that transcript and protein levels of rbcL in G.lemaneiformis were inhibited by heat stress(33℃),however,100μmol/L salicylic acid(SA100)and 50μmol/L methyl jasmonate(MJ50)treatments reduced the inhibition of high temper-ature on the expression of rbcL.In SA100 and MJ50 groups,the transcriptional expression of rbcL increased to 1.31-and 1.32-fold at 3 h,and the protein levels of rbcL were enhanced to 1.36-and 2.10-fold at 24 h compared to the heat-stress group,respectively.Moreover,SA100 and MJ50 effectively alleviated the inhibition effect of heat stress on Rubisco activity,and recovered the Rubisco activation states.However,the addition of 50μmol/L abscisic acid(ABA50)mainly inhibited rbcL expression and Rubisco activity.Heat stress stimulated the transcript and protein levels of HSP70;hsp70 transcript expression further rose by 0.53 1.00 times at 3 h,and its protein expression enhanced by 0.93 2.45 times at 24 h in the heat-stress group after applying SA100,MJ50 or ABA50.In conclusion,SA,MJ and ABA play a certain role in regulating the inhibition of photosynthesis enzyme and the induction of heat shock proteins caused by heat stress.
作者
吕燕
汪芳俊
林丽春
徐年军
陆开形
孙雪
Lü Yan;WANG Fangjun;LIN Lichun;XU Nianjun;LU Kaixing;SUN Xue(School of Marine Sciences,Ningbo University,Ningbo 315211,China;College of Science and Technology,Ningbo University,Ningbo 315211,China)
出处
《水产学报》
CAS
CSCD
北大核心
2019年第4期886-894,共9页
Journal of Fisheries of China
基金
国家自然科学基金(41376151
31672674)
宁波大学"水产"浙江省重中之重学科开放基金(xkzsc1525)~~
关键词
龙须菜
热休克蛋白70
Rubisco大亚基
高温
水杨酸
茉莉酸甲酯
脱落酸
Gracilariopsis lemaneiformis
heat shock protein 70
Rubisco large subunit
heat stress
salicylic acid
methyl jasmonate
abscisic acid
